| 桑叶多糖PMP12的分离纯化及结构初步分析 |
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| 引用本文： | 赵明,常钰,王佩香,张磊,欧阳臻.桑叶多糖PMP12的分离纯化及结构初步分析[J].江苏大学学报(医学版),2010,20(2):153-156. |
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| 作者姓名： | 赵明 常钰 王佩香 张磊 欧阳臻 |
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| 作者单位： | 1. 江苏大学药学院,江苏,镇江,212013;江苏大学附属医院临床药物试验机构,江苏,镇江,212001
2. 江苏大学药学院,江苏,镇江,212013 |
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| 基金项目： | 江苏省农业科技攻关项目，镇江市新产品新技术开发项目 |
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| 摘 要： | 目的:从桑叶中分离纯化获得均一多糖PMP12,并研究其组成及初步结构.方法:桑叶经热水提取乙醇分级沉淀,脱蛋白、脱色,经DEAE-纤维素和Sephadex G-100凝胶柱层析,得到一个均一多糖组分PMP12,采用气相(GC)、高效液相(HPLC)、红外(IR)、核磁(NMR)、Smith 降解和糖醛酸还原等方法分析其组成和初步结构.结果:PMP12由鼠李糖(Rha)、阿拉伯糖(Ara)、半乳糖(Gal)和葡萄糖醛酸(GluA)组成,其摩尔比为Rha:Ara:Gal:GluA=1:1.56:1.57:1.08;PMP12主链主要是以β-1→3糖苷键连接的鼠李糖,侧链主要是以β-1→2糖苷键及β-1→4糖苷键连接的阿拉伯糖、半乳糖和葡萄糖醛酸.结论:测定分析了桑叶多糖PMP12的组成和初步结构,为桑叶多糖的进一步研究提供依据.
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| 关 键 词： | 桑叶 多糖 分离纯化 结构分析 |
Separation,purification and preliminary structure analysis of polysaccharide PMP12 from Mulberry leaves |
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| ZHAO Ming,CHANG Yu,WANG Pei-xiang,ZHANG Lei,OUYANG Zhen.Separation,purification and preliminary structure analysis of polysaccharide PMP12 from Mulberry leaves[J].Journal of Jiangsu University Medicine Edition,2010,20(2):153-156. |
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| Authors: | ZHAO Ming CHANG Yu WANG Pei-xiang ZHANG Lei OUYANG Zhen |
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| Institution: | ZHAO Ming1,2,CHANG Yu1,WANG Pei-xiang1,ZHANG Lei1,OUYANG Zhen1(1.School of Pharmacy,Jiangsu University,Zhenjiang Jiangsu 212013,2.Clinical Drug Trial Institution,the Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001,China) |
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| Abstract: | Objective:To study the isolation,the composition and characterization of polysaccharide PMP12 from Mulberry leaves.Methods:Mulberry leaves were extracted by boiling water.The polysaccharide in the filtrate was precipitated fractionally by alcohol.The protein in the product was removed by TCA and it was further purified by DEAE ion exchange cellulose (DEAE-52)and SephadexG-100,received PMP12.The composition and characterization of Mulberry leaves polysaccharide were researched by GC,HPLC,IR,1H-NMR,Smith degr... |
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| Keywords: | Mulberry leaves polysaccharide separation and purification structure analysis |
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