Caspase-3活性改变对胆道梗阻大鼠中性粒细胞凋亡的影响

目的探讨半胱天冬酶-3(Caspase-3)活性的改变对胆道梗阻(BDO)大鼠外周血中性粒细胞(PMN)凋亡的影响。方法 54只SD大鼠随机分为正常组(A组)、假手术组(B组)和胆总管结扎组(C组),B、C组术后又分为1、3、7、10 d等4个时相,每个时相6只。留取血样标本分离PMN,应用流式细胞仪检测PMN凋亡率,采用荧光分光光度法检测Caspase-3活性。结果 C组PMN凋亡率从术后1 d的54.34±2.35降至10 d的36.01±2.11,低于A组(65.53±2.25)及B组相应时相,P<0.05;Caspase-3活性从术后1 d的52.33±2.35逐步下降至10 d的34.14±3.63,低于A组(60.58±5.35)及B组相应时相,(P<0.05)。结论 BDO大鼠外周血Caspase-3活性降低,可能导致PMN凋亡的减少,在梗阻性黄疸过度炎症反应和脓毒血症的发生、发展中具有重要意义。

Altered caspase-3 activity results in delayed polymorphonuclear neutrophil apoptosis in rats with bile duct obstruction

Objective To investigate the underlying mechanisms involved in the alteration of caspase-3 activity on peripheral polymorphonuclear neutrophil(PMN) apoptosis in rats with bile duct obstruction(BDO).Methods 54 SD adult rats were divided into three groups at random: normal rats termed Group A,other rats underwent either sham-ligated operation or bile duct obstruction termed Group B or Group C.Subsequently,Group B and Group C were randomly separated into subgroups of day 1,3,7,and 10.Blood samples were collected,PMN apoptosis was evaluated by flow cytometry and caspase-3 activity was detected by fluorescence staining.Results Group C displayed significantly decreased apoptosis of PMN from day 1(54.34±2.35) to day 10(36.01±2.11),as well as attenuated activity of caspase-3 on PMN from day 1(52.33±2.35) to day 10(34.14±3.63),when compared to group A(65.53±2.25),(60.58±5.35) and each subgroup B(P<0.01).Conclusion BDO rats reveal attenuated activity of caspase-3,which take part in regulation on PMN apoptosis process.Delayed PMN apoptosis may contribute to the excessive inflammation and severe septic complications,which plays an important role in the initiation and development of obstructive jaundice.