异丙肾上腺素对脂多糖诱导的大鼠肺泡巨噬细胞分泌TNF-α和IL-10及吞噬功能影响的研究

目的本实验在细胞水平研究异丙肾上腺素（ISO）对脂多糖（LPS）诱导的SD大鼠肺泡巨噬细胞（AM）分泌TNF-α及IL-10的影响。方法分离SD大鼠肺泡巨噬细胞，调节浓度为1×10^6。分组如下：①空白组，②LPS（10μg/ml）组，③ISO组，④LPS＋ISO组。以异丙肾上腺素（浓度分别为10μmol／L、100μmol／L、300μmol／L）加入SD大鼠肺泡巨噬细胞培养液。2h后，用脂多糖（10μg／ml）刺激，孵育6h后，以酶联免疫法测定上清中TNF-α，24h后，测定上清中IL—10的含量。结果与对照组相比，ISO组没有明显变化，LPS组TNF-α及IL-10的活性显著增加，10μmol／L、100μmol／L、300μmol／L ISO均能显著降低LPS诱导的大鼠肺泡巨噬细胞释放TNF—α，但是能促进LPS诱导的大鼠肺泡巨噬细胞释放IL-10，且呈剂量依赖性，并且能增加巨噬细胞对中性红的吞噬能力。结论异丙肾上腺素能调节巨噬细胞分泌功能，使SD大鼠肺泡巨噬细胞减少炎性因子TNF-α分泌，增加抑炎因子IL-10分泌．增强巨噬细胞的吞噬中性红的能力。

Study of Isoprenaline on effect of secreting TNF-α and IL-10 and phagocytizing of LPS-introduced alveolar macrophages in rats

Objective This study was to observe the effect of Isoprenaline on lipopolysacchride (LPS)-induced secreting of TNF-α and IL-10 in Alveolar Macrophages (AM) of SD rats. Methods AM were isolated from normal rats and adjusted to 1×106 / ml and incu- bated in the following groups: ①Control group; ②L PS group; ③ISO group:10μM, 100μM, and 300μM in respectively;④LPS+ ISO group: L PS 10μg/ ml; ISO: 10μM, 100μM, and 300μM. Isoprenaline at various concentrations (10 μmol/l, 100 μmol/l, 300 μmol/l) were added in AM cells culture fluid, then stimulated with 10ug/ml LPS after 2 hours, the supernatants were collected for measuring TNF-α and IL-10 by ELISA kit after 6h and 24 h respectively. Resealts Compared with Control group, ISO group has no change, but 10ug/ml LPS significantly induced the production of TNF-α and IL-10, When Isoprenaline in combination with LPS was added into the medium, the TNF-α production was suppressed and the IL-10 production was increased in a dose-dependent manner Isoprenaline could increase the capability of phagocytizing neutral red. Conclusion Isoprenaline down-regulates LPS-induced pro-inflammatory mediators production in macrophage, but up-regulates LPS-induced anti-inflammatory mediators production. Isoprenaline increases the capability of phagocytizing neutral red.