Establishment of human myeloma cell line KM-2R and its preliminary application to human-human hybridoma research. |
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| Authors: | Y Guo H Zhang Y Fan K Lin C Gong J Fu R Guo |
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| Institution: | Institute of Medical Biology, CAMS, Kunming. |
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| Abstract: | The authors report the establishment of a human myeloma cell line--KM-2R--and its preliminary use in human-human hybridoma research. KM-2R cells are resistant to both 6-TG and ouabain. Their doubling time is about 30 h. They have a modal chromosome number of 81-85. Using ELISA and immunodiffusion techniques, gamma-heavy and kappa-light chains were detected in the concentrated cell culture supernatant. KM-2R cells are sensitive to HAT medium. When fusing with normal human spleen cells, tonsil cells, and B lymphoblasts transformed by EB virus, fusion frequencies of 20-60% resulted. |
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