| SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS |
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| 引用本文： | 刘新平,陈苏民,陈南春,高磊,赵忠良.SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN （2A） WITH MOUSE OSTEOBLASTIC CELLS[J].中国医学科学杂志,1996,11(2):97-99. |
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| 作者姓名： | 刘新平 陈苏民 陈南春 高磊 赵忠良 |
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| 作者单位： | TheFourthMilitaryMedicalUniversity,InstituteofMolecularBiology,Xi＇an710032 |
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| 摘 要： | Human bone morphogenetic protein 2A (hBMP2A) cDNA terminal 567 nucleotides were cloned and expressed in a phage display vector pCSM2I. Hulnata BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the sttrface of mouse osteoblastie cell (MC3T3) membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with ^3HTdR,the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the‘surface of MC3T3 cells exist the recepzor for hBMP2A.
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| 关 键 词： | 骨形成蛋白 造骨细胞 动物实验 克隆 pCSM2I ELISA TGF |
SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN (2A) WITH MOUSE OSTEOBLASTIC CELLS |
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| Liu Xinping, Chen Sumin, Chen Nanchun, Gao Lei, and Zhao Zhongliang,.SPECIFIC BINDING OF HUMAN BONE MORPHOGENETIC PROTEIN (2A) WITH MOUSE OSTEOBLASTIC CELLS[J].Chinese Medical Sciences Journal,1996,11(2):97-99. |
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| Authors: | Liu Xinping Chen Sumin Chen Nanchun Gao Lei and Zhao Zhongliang |
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| Abstract: | Human bone morphogenetic protein 2A (hBMP2A) cDNA terminal 567 nucleotides were cloned and expressed in a phage display vector pCSM21. Human BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the surface of mouse osteoblastic cell (MC3T3) membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with 3HTdR,the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the surface of MC3T3 cells exist the receptor for hBMP2A. |
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| Keywords: | human bone morphogenetic protein 2A ELISA 3HTdR incorporation |
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