| CLONING AND CHARACTERIZATION OF A METAL RESPONSIVE ELEMENT-CONTAINING FRAGMENT FROM THE WILSON DISEASE GENE LOCUS BY JUNCTION TRAPPING |
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| 作者姓名： | 谢久永 刘国仰 王梅 黄尚志 罗会元 |
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| 作者单位： | DepartmentofMedicalGenetics,InstituteofBasicMedicalSciences,CAMS＆PUMC,Beijing100005 |
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| 基金项目： | The sequence described in this paper has been deposited in Gen-Bank with the accession number U90431. This work was
supporte |
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| 摘 要： | All mammalian metallothionaln genes studied to dare have several metal responsive elements (MRE) with consensus sequences of TGCRCNC (R, purlne) in their regulatory region. MRE-11ke sequeaees were also found in many other metal-related genes. To see whether there is also such a sequence at the genetic locus (13q14. 3) d Wilstm disease, which is a genetic disorder d copper metabolisa‘‘n, junction-trapping method baaed on the MRE sequence was used. A fragment containing MRE and MRE-like sequences from YAC 27D8 at the WND locus was successfully cloned and mapped back to the YAC by PC, R, Presence of such a sequence in the copper transporter gene at the W‘‘D locus might imply that it has a possible interesting role in the regulation of WD gene expression.
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| 关 键 词： | 进行性肝豆状核变性 金属应答元素 结型吸引 基因定位 克隆 |
CLONING AND CHARACTERIZATION OF A METAL RESPONSIVE ELEMENT-CONTAINING FRAGMENT FROM THE WILSON DISEASE GENE LOCUS BY JUNCTION TRAPPING |
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| Xie Jiuyong,Liu Guoyang,Wang Mei,Huang Shangzhi and LoWilson H. Y..CLONING AND CHARACTERIZATION OF A METAL RESPONSIVE ELEMENT-CONTAINING FRAGMENT FROM THE WILSON DISEASE GENE LOCUS BY JUNCTION TRAPPING[J].Chinese Medical Sciences Journal,1998,13(1):9-13. |
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| Authors: | Xie Jiuyong Liu Guoyang Wang Mei Huang Shangzhi and LoWilson H Y |
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| Institution: | Department of Medical Genetics, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005. |
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| Abstract: | All mammalian metallothionein genes studied to date have several metal responsive elements (MRE) with consensus sequences of TGCRCNC (R, purine) in their regulatory region. MRE-like sequences were also found in many other metal-related genes. To see whether there is also such a sequence at the genetic locus (13q14. 3) of Wilson disease, which is a genetic disorder of copper metabolism, junction-trapping method based on the MRE sequence was used. A fragment containing MRE and MRE-like sequences from YAC 27D8 at the WND locus was successfully cloned and mapped back to the YAC by PCR. Presence of such a sequence in the copper transporter gene at the WD locus might imply that it has a possible interesting role in the regulation of WD gene expression. |
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| Keywords: | metal responsive element (MRE) Wilson disease junction-trapping |
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