| IDENTIFICATION OF GLUCOSE TRANSPORTER－1 AND ITS FUNCTIONAL ASSAY IN MOUSE GLOMERULAR MESANGIAL CELLS CULTURED IN VITRO |
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| 引用本文： | 章精,刘志红,刘栋,黎磊石.IDENTIFICATION OF GLUCOSE TRANSPORTER－1 AND ITS FUNCTIONAL ASSAY IN MOUSE GLOMERULAR MESANGIAL CELLS CULTURED IN VITRO[J].中国医学科学杂志,2001,16(1):35-39. |
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| 作者姓名： | 章精 刘志红 刘栋 黎磊石 |
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| 作者单位： | ResearchInstituteofNephrology,JinglingHospital,NanjingUniversitySchoolofMedicine,Nanjing210002 |
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| 摘 要： | Objective. To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesanglal cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-3H] -D-glucose uptake. Results. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. Conclusion. Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells.
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| 关 键 词： | 糖尿病肾病 肾小球系膜细胞 葡萄糖载体 荧光免疫试验 血细胞计数 |
Identification of glucose transporter-1 and it's functional assay in mouse glomerular mesangial cells cultured in vitro. |
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| J Zhang,Z Liu,D Liu,L Li.Identification of glucose transporter-1 and it''s functional assay in mouse glomerular mesangial cells cultured in vitro.[J].Chinese Medical Sciences Journal,2001,16(1):35-39. |
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| Authors: | J Zhang Z Liu D Liu L Li |
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| Institution: | Research Institute of Nephrology, Jingling Hospital, Nanjing University School of Medicine, Nanjing 210002. |
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| Abstract: | OBJECTIVE: To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesangial cells. METHODS: Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-3H] -D-glucose uptake. RESULTS: Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. CONCLUSION: Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells. |
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