增生为主IgA肾病阴虚证与TGF—β1基因启动子区C-509T的相关性研究

目的观察增生为主原发性IgA肾病（IgAN）患者阴虚证与转化生长因子β1（TGF—β1）基因启动子区C-509T的相关性。方法筛选符合标准的原发性IgAN患者120例，进行TGF—β1基因启动子区C-509T的单核苷酸多态性（SNP）测序。观察中医证型与SNP的相关性。结果120例患者中TGF-β1基因启动子区C-509T位点中TT型28例，TC型54例，CC型38例。通过对TGF-β1基因启动子区C-509T与增生为主原发性IgAN患者气阴两虚证、肝肾阴虚证相关性研究，发现肝肾阴虚证在CC组中所占比例相对较高，气阴两虚在TT＋CT组中所占比例相对较高（P〈0．01，CC型与CT＋TT型OR值3．977，95％CI：1．771～8．831）。这样的差异同样存在于不同性别、不同年龄之间。结论TGF—β1基因启动子区C-509T可能是区分原发性IgAN肝肾阴虚证、气阴两虚证的物质基础之一。

Correlation between TCM Syndromes to Primary IgA Nephropathy Mainly Manifesting as Hyperplasia and Polymorphism C-509T in Promoter Region of the TGF -β1 Gene

Objective： Objective： To observe the correlation between TCM syndromes to primary IgAN manifesting as hyperplasia and polymorphism C -509T in promoter region of the TGF -β1 gene. Method： A total number of 120 patients meeting diagnostic criteria were enrolled in order to test the sequence of polymorphism C - 509T in promoter region of the TGF -β1 gene. The correlation between SNP and TCM syndromes was observed. Results： There were 28 cases of TT genotype, 54 cases of CT genotype and 38 cases of CC genotype in the total 120 cases of primary IgAN. Through the comparison between ＂deficiency of both qi and yin＂ and ＂deficiency of both liver yin and kidney yin＂ to primary IgAN mainly manifesting as hyperplasia distributing the polymorphism C -509T in promoter region of the TGF -β1 gene, it was demonstrated that there was a higher proportion of ＂deficiency of both liver yin and kidney yin＂ in CC Group and higher proportion of ＂deficiency of both qi and yin＂ in .TT and CT Group （ P 〈 0.01, CC genotype versus CT ＋ TT genotype, OR = 3. 977, 95% CI = 1. 771 ～ 8. 931 ）. The discrepancy also resides in different gender and age. Conclusion： The polymorphism C -509T in promoter region of the TGF -β1 gene could be one of the substantial things which can differentiate ＂deficiency of liver yin and kidney yin＂ and ＂ deficiency of.both qi and yin＂ to primary IgAN.