| 清脑灵诱导大鼠骨髓间充质干细胞分化成神经元样细胞 |
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| 引用本文： | 王金良,薛卡明,刘建国,王岚,谢仪凤.清脑灵诱导大鼠骨髓间充质干细胞分化成神经元样细胞[J].湖北中医学院学报,2010,12(2):12-14. |
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| 作者姓名： | 王金良 薛卡明 刘建国 王岚 谢仪凤 |
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| 作者单位： | 华中科技大学同济医学院;华中科技大学同济医学院附属协和医院中医科; |
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| 摘 要： | 目的观察清脑灵煎剂是否能诱导大鼠骨髓间充质干细胞(MSCs)分化成神经元样细胞。方法通过贴壁法分离获得大鼠骨使髓间充质干细胞,体外扩增培养,用流式细胞仪检测细胞表面抗原的表达。用浓度为1mg/mL的清脑灵煎剂诱导MSCs,分别于5h、12h、1d、3d、7d在倒置显微镜下观察细胞形态变化,用免疫细胞化学方法观察被诱导细胞巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经胶质纤维酸性蛋白(GFAP)的表达。结果流式细胞仪检示MSCs不表达CD11b、CD45,诱导后细胞的免疫细胞化学示nestin阳性细胞、NSE阳性细胞和GFAP阴性细胞。结论清脑灵能诱导MSCs分化神经元样细胞。
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| 关 键 词： | 清脑灵 骨髓问充质干细胞 神经元样细胞 |
Qingnaoling Inducing Rat Bone mMesenchymal Stem Cells(MSCs) Differentating to Neuron-like Cells |
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| WANG Jinliang,XUE Kaming,LIU Jianguo,WANG Lan,XIE Yifeng.Qingnaoling Inducing Rat Bone mMesenchymal Stem Cells(MSCs) Differentating to Neuron-like Cells[J].Journal of Hubei College of Traditional Chinese Medicine,2010,12(2):12-14. |
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| Authors: | WANG Jinliang XUE Kaming LIU Jianguo WANG Lan XIE Yifeng |
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| Institution: | 1.Grade 2007 Postgraduate Student of College of Tongji Medical College of Huazhong University;Wuhan 430022;Hubei;2.Dept.of TCM;Wuhan Union Hospital Affiliated to College of Tongji Medical College of Huazhong University of Science and Technology;Hubei |
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| Abstract: | Objective To investigate if Qingnaoling decoction was able to induce rat bone mesenchymal stem cells(MSCs) differentating to neuron-like cells.Methods MSCs were separated and got from rat bone marrow with adheresion method,amplified and cultured in vitro,surface antigens expression was detected by flow cytometry(FCM).MSCs were induced by 1mg/ml Qingnaoling decoction and observed after 5 hours,12 hours,1day,3days,7days under inverted microscope for cellular morphology changes.Nestin,neuron-specific enolase(N... |
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