在大鼠胃壁注射阿霉素治疗胃顽固性痛的研究

目的通过动物实验探讨一种胃顽固性痛的镇痛方法。方法Wister大鼠30只，随机平均分为对照组（A组）和实验组（B组）。按实验设计处理，第3天，取双侧腹腔神经节、T6-9背根神经节、双侧T6-9脊神经前根及脊髓观察阿霉素在上述组织中的荧光表达；第8周末，胃内灌注甲醛溶液，观察各组大鼠的疼痛反应。2h后灌注固定，取脊髓行c-fos免疫组织化学染色，观察脊髓灰质背角c-fos表达；取双侧腹腔神经节、T6-9背根神经节及心脏和胃注射区行HE染色，观察其形态学变化。结果荧光表达：A组各部位均无荧光表达，B组双侧T6-9背根经节均有橘红色颗粒状荧光表达；双侧腹腔神经节内见穿经该神经节的内脏感觉神经纤维外膜有荧光表达；A组呼吸频率、脊髓灰质后角部位c-fos光密度值明显高于B组（P〈0．05）；HE染色：双侧T6-9背根神经节部分神经元胞体消失，神经胶质细胞增生。腹腔神经节内神经元胞体未见损伤。结论胃壁注射浓度1％的阿霉素，可有效治疗大鼠胃病，并保持胃交感神经的完整性，有效地保护背根神经节内的躯体感觉神经元，保持了T6-9神经相应分布区的躯体感觉功能，是一种有效的胃顽固性疼痛治疗方法。

Study of Treatment of Gastric Intractable Pain with Adriamycin Injected in the Rat Stomach

Objective Approach to treatment of gastric intractable pain through the animal experiments. Methods Thirty Wister rats were randomly divided into the control group （ group A ） and the experimental group （ group B）. The bilateral organization of celiac ganglion, T6-9 dorsal root ganglia, ventral root of spinal nerves and spinal cord were taken . Observing adriamycin fluorescence in the above-mentioned organization after 3 days. After 8 weeks, Rats＇ pain reaction in different groups were observed when formalin was poured into the rats＇stomach. The spinal cord was stained by c-fos immunohistochemistry and the expression of c-fos in dorsalhorn of gray nucleus was observed. The morphological changes of celiac ganglion,T6-9 dorsal root ganglia,ventral root of spinal nerves, spinal cord,heart and stomach were observed under light microscope. Results The orange granular fluorescence was found in celiac ganglion and T6-9 dorsal root ganglia of group B but adriamycin fluorescence was not found in group A. Breathing frequency and c-fos light density of group A in the grey area of spinal cord was obviously higher than that of group B （ P 〈 0.05 ）. Some neurons of bilateral T6-9 dorsal root ganglia were disappeared while neuron of celiac ganglion was not damaged. Conclusion This method is an effective treatment for stomach intractable pain which can effectively keep the integrity of stomach sympathetic and the T6-9 nerve distribution function of somatesthesia.