Inhibitive effect of loureirin B plus capsaicin on tetrodotoxin-resistant sodium channel |
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Authors: | Chen Su Wan Ying Liu Xiangming Pan Xinxin |
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Institution: | 1. College of Biomedical Engineering, South-Central University for Nationalities, Wuhan 430074, China;2. Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment, Wuhan 430074, China;3. Key Laboratory of Cognitive Science of State Ethnic Affairs Commission, Wuhan 430074, China;4. College of Biomedical Engineering, South-Central University for Nationalities, Wuhan 430074, China;5. GongQing Institute of Science and Technology, Jiujiang 332020, China;6. College of Biomedical Engineering, South-Central University for Nationalities, Wuhan 430074, China |
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Abstract: | OBJECTIVETo investigate whether the effect of loureirin B plus capsaicin on tetrodotoxin-resistant (TTX-R) sodium channel.METHODSBy using whole-cell patch-clamp recordings, in acutely isolated dorsal root ganglion (DRG) neurons, the combined effects of loureirin B and capsaicin on TTX-R sodium channel were observed. Based on the data, the interaction between loureirin B and capsaicin in their modulation on TTX-R sodium channel was assessed.RESULTSLoureirin B could not induce transient inward TRPV1 current. Capsazepine, a transient receptor potential vanilloid l (TRPV1) antagonist, could not attenuate the block of 0.64 mmol/L loureirin B on TTX-R sodium channel. There was no significant difference (P > 0.05) between IC50 of loureirin B (0.37 mmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (0.38 mmol/L) in capsaicin-insensitive DRG neurons. However, there was a significant difference (P < 0.05) between the IC50 of capsaicin (0.28 μmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (52.24 μmol/L) in capsaicin-insensitive DRG neurons. Four combinations composed of various concentrations of loureirin B and capsaicin could all inhibit TTX-R sodium currents but have different interactions between loureirin B and capsaicin.CONCLUSIONLoureirin B plus capsaicin could produce double blockage on TRPV1 and modulation on TTX-R sodium channel. The action of loureirin B on TTX-R sodium channel was independent of TRPV1 but similar with that of capsaicin on TTX-R sodium channel in capsaicin-insensitive DRG neurons. |
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Keywords: | Loureirin B Capsaicin TRPV cation channels Spinal nerve roots Neurons Tetrodotoxin Sodium channels |
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