白藜芦醇对血管性痴呆模型大鼠学习和记忆能力的影响

目的：观察白藜芦醇对血管性痴呆（VD）模型大鼠学习和记忆能力的影响及其作用机制.方法：30只SD大鼠完全随机法分为模型组、假手术组和实验组,每组10只.模型组大鼠在腹腔注射硝普钠降低血压的基础上,反复夹闭、再通双侧颈总动脉制备VD大鼠模型,术后第2天给予生理盐水灌胃,连续28 d;假手术组大鼠手术过程与模型组相同,但术中不注射硝普钠、不阻断双侧颈总动脉,术后处理同模型组;实验组大鼠手术过程与模型组相同,术后第2天给予白藜芦醇溶液灌胃,连续28 d.造模后第21天进行Y-迷宫测试、造模后第28天进行Morris水迷宫测试,检测各组大鼠学习记忆能力,并测定大鼠大脑皮层和海马组织中一氧化氮合酶（NOS）、一氧化氮（NO）含量和谷胱甘肽过氧化物酶（GSH-PX）活性.结果：与假手术组比较,模型组大鼠学习和记忆能力明显下降;大脑皮层及海马组织NOS及NO含量升高,GSH-PX活性下降（均P〈0.05）.与模型组比较,实验组大鼠学习、记忆能力提高;大脑皮层及海马组织NOS及NO含量下降,GSH-PX活性升高（均P〈0.01）.结论：白藜芦醇对VD模型大鼠学习记忆能力具有一定改善作用,可能通过提高大鼠脑组织的抗氧化能力而发挥保护作用.

Effect of resveratrol on learning and memory ability in rats with vascular dementia

Objective：To investigate the effect of resveratrol （Res） on learning and memory ability in rats with vascular dementia （VD） and its mechanism. Methods：Thirty rats were randomly divided into model group, sham-operated group and experiment group. VD rat models were established by repeated clamping and opening of bilateral common carotid arteries added on intraoperative hypotension induced by intraperitoneal injection of sodium nitroprusside. The sham-operated rats underwent the same procedure without manipulations on bilateral common carotid arteries and injection of sodium nitroprnsside. Starting from day 2 after operation, the rats received gavage of normal saline （VD model group and sham-operated group） or Res（ experiment group） for a total of 28 days. The learning and memory ability in all rats was observed with Y-maze and water maze tests on day 21 and 28 after operation. The contents of NOS and NO, and the activities of glutathione peroxidase （ GSH-PX） in rats cerebral cortex and hippocampus were measured. Results： Compared wilh sham-operated group,the VD models showed lower learning and memory abilities, significantly increased contents of NOS and NO, and reduced activities of GSH-PX in the cerebral cortex and hippocampus （all P 〈0.05）. In the experiment group, the learning and memory ability was enhanced, NOS and NO content decreased and the activity of GSH-PX increased,as compared remarkably with findings from the model group （all P 〈 0.01 ）. Conclusion： Res may improve the learning and memory ability in VD model rats, which may be related to increased antioxidation ability in the brain tissue.