内皮抑素基因联合小剂量阿霉素对裸鼠乳腺癌的治疗

目的：探讨鼠原性内皮抑素（Es）联合小剂量阿霉素治疗裸鼠乳腺癌的疗效。方法：构建逆转录病毒载体质粒DFG—ESDNA，建立乳腺癌裸鼠模型，将肿瘤生长均匀的裸鼠分成对照组、ES组、小剂量阿霉素组、ES与小剂量阿霉素联合治疗组。分别于肿瘤对侧背部皮下注射1X10^6（0．1mL）转染ES的人成纤维细胞，尾静脉注射阿霉素1mg／kg。每周测量1次肿瘤大小，根据V=0．52×L2XW计算体积。结果：ES与小剂量阿霉素联合治疗组肿瘤体积明显小于其他各组（P〈0．01），细胞凋亡率明显高于其他各组（P〈0．01）。结论：ES可以有效抑制肿瘤血管生成，抑制裸鼠乳腺癌的生长，与小剂量阿霉素联合应用可明显增强其抗乳腺癌生长的疗效，为今后探索乳腺癌治疗提供新途径。

Combination of endostatin gene with doxorubicin in nude mice with breast cancer

Objective： To study the efficacy of the combination of mouse endostatin gene （ES） with a small dose of doxorubicin in nude mice with breast cancer. Methods： Retrovira] vector plasmid endostatin （ DFG- ESDNA） was constructed and breast cancer mice models were established. The mice with well-distributed tumors were randomized into control group, endostatin（ES） group, small dose of doxorubicin group, endostatin and small dose of doxorubicin group. Endostatin-transfected human fibroblasts at a volume of 1 × 10^6 （0. 1 mL） were subcutaneously inoculated and doxorubicin at 1 mg/kg was injected into the tail vein. Tumor size was measured once a week, with volume computed under V = 0.52 × L2 × W. Results ： In endostatin and doxorubicin group, tumor volume decreased much faster than other groups （P 〈 0.01 ） , besides, the apoptosis rate of tumor cells was significantly higher （P 〈 0.01 ）. Conclusion： Endostatin gene may effectively suppress tumor angiogenesis and inhibit progression of breast cancer in mice. The combination of ES with doxorubiein significantly enhances the efficacy in combating breast cancer, which may be a possible novel therapeutic strategy for breast cancer in future.