电针对急性脑缺血大鼠血清NO、NOS、ET-1的影响

目的探讨电针对急性脑缺血的大鼠作用机制。方法将60只大鼠随机分为5组,即:空白组,假手术组,模型组,模型 电针人中、内关组(中内模型组),模型 电针曲池、足三里组(曲足组)。每组12只大鼠,随机分为甲、乙两小组。采用改良Longa线栓法制作大鼠急性脑缺血再灌注损伤模型。甲组大鼠在24 h时处死,乙组大鼠在48 h时间点处死。采颈动脉血检测血清中NO、NOS和ET-1的含量。结果与空白组比较,模型组、中内模型组、曲足组大鼠血清NO、NOS、ET-1升高,差异有统计学意义(P<0.05)。中内模型组血清中NO、NOS、ET-1的含量明显低于模型组,且差异具有统计学意义(P<0.05)。结论电针能抑制NO、NOS、ET-1的过度升高,可能是电针中内模型组对急性脑缺血大鼠的作用机制之一。

Effect of electroacupuncture on NO, NOS and ET-1 in serum of rats with acute cerebral ischemia

Objective To investigate the pre-protective mechanism of electroacupuncture on acute cerebral ischemia.Methods 72 rats were randomly divided into six groups,namely blank,sham-operated,model,electroacupuncture renzhong and neiguan(ERN),model with electroacupuncture renzhong and neiguan(MERN),and model with electroacupuncture quchi and zusanli(MEQZ) groups,12 rats in each group,all the groups were randomly divided into A and B group again.The models of rats with acute cerebral ischemia were induced a ccording to modified thread pin of Longa.All rats in group A were killed after 24 hours,and in group B after 48 hours,then,the content of NO,NOS and ET-1 in serum were determined.Results The content of NO,NOS and ET-1 in serum in MERN decreased more obviously than that in model group(P<0.05).Conclusion The expression of NO,NOS and ET-1 in serum are Inhibited excessively which may be one of the pre-protective mechanism of electroacupuncture on acute cerebral ischemia.