愈痫灵方对PTZ致痫大鼠脑组织海马CA3区TLR4、NF-kB P65、TNF-α表达的影响

目的 探讨愈痫灵方对痫性大鼠脑组织海马区炎性反应因子表达的影响.方法 取健康雄性SD大鼠120只,从中随机选取10只作为正常对照组,10只作为假手术组,腹腔注射生理盐水;其余大鼠用戊四氮造模,将符合模型标准的大鼠随机分为愈痫灵组、丙戊酸钠组和模型组,每组10只.灌胃后采用免疫组织化学方法检测TLR4、NF-kB P65和TNF-α在海马CA3区的表达变化.结果 与正常组、假手术组比,模型组、愈痫灵方组、丙戊酸钠组均上调TLR4、NF-kB P65和TNF-α在脑组织海马CA3区的表达(P<0.01),但后两组表达均低于模型组(P<0.05),且愈痫灵方组抑制TLR4和NF-kB P65在脑组织海马CA3区表达的作用优于丙戊酸钠组(P<0.05),而TNF-α在脑组织海马CA3区的表达两组间无明显差异(P>0.05);结论 愈痫灵方可抑制PTZ致痫大鼠脑组织海马CA3区TLR4、NF-kB P65和TNF-α的表达,对癫痫反应中炎性因子的表达有一定的抑制作用.

Effects of Yuxianling Decoction on TLR4, NF-kB P65, TNF-α Expressions in Brain Hippocampal CA3 Region of Epileptic Rats Induced by PTZ

Objective To investigate the effect of Yuxianling Decoction on the rats' brain tissue inflammatory factor expression. Methods 120 healthy male SD rats were selected, 10 rats were randomly chosen as a normal control group, 10 rats as the sham-operation group, with normal saline injected in the belly. The other rats were treated with Corazol, and divided the rats that met the standard into Yuxianling group, sodium valproate group, and model group, with 10 rats in each group. The expression of TLR4, NF-kB P65 and TNF-α in brain hippocampal CA3 region were detected by immunohistochemistry. Results Compared with the normal group and sham-operation group, the expression of TLR4, NF-kB P65 and TNF-α of the model group, Yuxianling Decoction group, and the sodium valproate group in hippocampal CA3 region upregulated (P<0.01), while the latter two groups were lower than that of model group (P<0.05), and Yuxianling Decoction group was better than that of sodium valproate group in inhibiting TLR4 and NF -kB P65 expression in hippocampal CA3 region (P<0.05). However, there is no significant differences between the two groups in the expression of TNF-α in brain tissue (P>0.05). Conclusion Yuxianling decoction can inhibit the TLR4, NF-kB P65 and TNF-α expression in hippocampal CA3 region of epileptic rats induced by PTZ and it has certain inhibitory effect on the expression of inflammatory factor in epilepsy.