| HPLC法测定三叶海棠叶中白杨素苷含量 |
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| 引用本文： | 江星明,肖美凤,彭新君,文礼章.HPLC法测定三叶海棠叶中白杨素苷含量[J].湖南中医药大学学报,2009,29(2):43-45. |
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| 作者姓名： | 江星明 肖美凤 彭新君 文礼章 |
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| 作者单位： | 1. 湖南中医药大学药学院,湖南,长沙,410208
2. 湖南农业大学生物安全科学技术学院,湖南,长沙,411007 |
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| 基金项目： | 湖南省卫生厅资助项目 |
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| 摘 要： | 目的建立三叶海棠叶中白杨素苷含量测定方法。方法采用高效液相色谱法测定，色谱柱：Gemini C18 110Aa（150mm×4．6mm，5μm），流动相：乙腈-水（30：70），流速：1．0mL/min，柱温：40℃，检测波长：270nm，进样量：20μL。结果白杨素苷在2．0—20．0μg／mL范围内具有良好的线性关系（r=0．9995），三叶海棠叶中白杨素苷的平均含量为5．05％，平均回收率为97.4％，RSD为1.2％（n=5）。结论三叶海棠叶中白杨素苷含量较高，该测定方法灵敏、结果准确、重现性好。
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| 关 键 词： | 三叶海棠叶 白杨素苷 紫外分光光度法 高效液相色谱法 |
Determination of Chrysin-7-glucoside in Leaf of Nalussieboldji with HPLC |
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| JIANG Xing-ming,XIAO Mei-feng,PENG Xin-jun,WEN Li-zhang.Determination of Chrysin-7-glucoside in Leaf of Nalussieboldji with HPLC[J].Journal of Traditional Chinese Medicine University of Hunan,2009,29(2):43-45. |
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| Authors: | JIANG Xing-ming XIAO Mei-feng PENG Xin-jun WEN Li-zhang |
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| Institution: | TCM University of Hunan;Changsha;Hunan 410208;China |
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| Abstract: | Objective To establish a method for the determination of chrysin-7-glucoside in Nalussieboldji. Method HPLC was used in the determination with Gemini C18 column（150 min×4.6 mm, 5 μm）, mobile phase of acetonitrile-water 30：70, flow rate 1.0 mL/min, column temperature 40℃ detection wavelengh 270 nm and injection volume 20 L. Results Chrysin-7- glucoside had a linear range in the contents of 2.0-20.0 g/mL （r=0.999 5）and the linear equation was Y=7.737×10^4X-6.0×1^3 in the range of 2.0-20.0 g/mL（r=0.999 5）. The average content of chrysin-7-glucoside in leaf of Nalussieboldji was 5.05% and the average recovery was 97.4% with RSD 1.2%（n=5）. Conclusion The method is simple, reliable and accurate for determination of chrysin-7-glucoside. |
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| Keywords: | Nalussieboldji chrysin-7-glucoside UV HPLC |
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