厄贝沙坦对巨噬细胞凝集素样氧化低密度脂蛋白受体(LOX-1)基因及蛋白表达的影响

目的研究不同浓度厄贝沙坦对血管紧张素Ⅱ(AngⅡ)诱导的人单核/巨噬细胞系(THP-1)凝集素样氧化低密度脂蛋白受体(LOX-1)mRNA和蛋白表达的影响,并探讨其可能的机制。方法 THP-1细胞经0.16μmoL/L佛波酯诱导分化后,将细胞分为3组:对照组、AngⅡ组、厄贝沙坦干预组,干预组分别加入不同浓度厄贝沙坦孵育2 h后,再加入AngⅡ1×10-6moL/L孵育24 h,用荧光定量PCR和细胞酶联免疫法检测(LOX-1)mRNA和蛋白表达。结果与对照组比较,Ang II可明显上调THP1细胞(LOX-1)mRNA和蛋白表达,差异有统计学意义(P<0.05)。不同浓度厄贝沙坦均能抑制(LOX-1)蛋白表达(P<0.05),并且随着厄贝沙坦浓度降低,抑制作用减低,即两者呈浓度依赖性。结论厄贝沙坦以浓度依赖方式抑制AngⅡ诱导的THP1细胞(LOX-1)mRNA和蛋白表达,减少巨噬细胞通过(LOX-1)途径的氧化低密度脂蛋白(oxLDL)摄入,影响泡沫细胞的发生、发展,发挥其抗动脉粥样硬化(AS)作用。

Effects of irbesartan on expression of lectin-like oxidized low-density lipoprotein receptor-1 mRNA and protein in cultured THP-1 cells

Objective To investigate the influence of irbesartan on expression of lectin-like oxidized low-density lipoprotein receptor-1 mRNA and protein in cultured THP-1 cells Methods THP-1 cells were differentiated by incubation with 160nM PMA for 72 hours.The cells were divided into three groups:control group,AngⅡgroup and irbesartan intervention group.The intervention groups were first treated with several concentration irbesartan for 2 hours,then coincubated with AngⅡ1×10-6moL/L for24h.Real time PCR and enzyme-linked immunosorbent assay technology were used to detect(LOX-1) mRNA and protein expression.Results AngⅡ induced the increase of THP-1(LOX-1) mRNA and protein expression(P<0.05).Several concentration irbesartan groups blocked AngⅡ-induced(LOX-1) mRNA and protein expression,The decrease in(LOX-1) expression was dependent on irbesartan concentration.Conclusion Irbesartan can blocke AngⅡ-induced(LOX-1) mRNA and protein expression in a concentration-dependent and reduce intake of macrophages on the oxidized low density lipoprotei via(LOX-1) pathway,affecting the foam cell development,exert their effect on anti-atherosclerosis.