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| 六味地黄方超微饮片含药血清对大鼠前脂肪细胞的影响 | |
| 引用本文： | 肖子曾,赵婧,戴冰,冷旺,梅君,李兴丰. 六味地黄方超微饮片含药血清对大鼠前脂肪细胞的影响[J]. 湖南中医药大学学报, 2010, 30(1): 22-24 |
| 作者姓名： | 肖子曾 赵婧 戴冰 冷旺 梅君 李兴丰 |
| 作者单位： | 1. 湖南中医药大学,湖南,长沙,410007 2. 湖南中医药大学硕士研究生班,湖南,长沙,410007 3. 湖南中医药大学第一附属医院,湖南,长沙,410007 |
| 基金项目： | 湖南省科技厅科研资助项目(2008FJ3071);;湖南省教育厅资助项目(06C610);;湖南省中医药管理局资助项目(6201)(2008099) |
| 摘 要： | 目的 探讨六味地黄方超微饮片含药血清对大鼠前脂肪细胞增殖与分化的影响。方法原代培养大鼠前脂肪细胞。用四甲基偶氮唑盐（MTT）方法检测六味地黄方超微饮片含药血清对前脂肪细胞的增殖情况。以酶组织化学法检测其对前脂肪细胞分化过程中的磷酸甘油脱氢酶（GPDH）的影响。油红O染色方法测定其对细胞内脂肪积聚的影响。结果10．0％、5．0％、2．5％浓度的六味地黄方超微饮片舍药血清对大鼠前脂肪细胞增殖的影响与空白血清比较，差异均有统计学意义（P〈0．05）。10．0％、5．0％浓度的六味地黄方超微饮片舍药血清对大鼠前脂肪细胞分化过程中脂肪积聚的影响，与空白血清比较，差异有统计学意义（P〈0．05）。结论六味地黄方超微饮片能促进大鼠前脂肪细胞的增殖，抑制大鼠前脂肪细胞的分化。 |
| 关 键 词： | 六味地黄方 超微饮片 含药血清 大鼠 前脂肪细胞 增殖 熟地黄 山茱萸 茯苓 |
Effects of drug-containing serum with Liuwei Dihuang micro-powder on preadipocyte in rats |
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| XIAO Zi-zeng,ZHAO Jing,DAI Bing,LENG Wang,MEI Jun,LI Xing-feng. Effects of drug-containing serum with Liuwei Dihuang micro-powder on preadipocyte in rats[J]. Journal of Traditional Chinese Medicine University of Hunan, 2010, 30(1): 22-24 | |
| Authors: | XIAO Zi-zeng ZHAO Jing DAI Bing LENG Wang MEI Jun LI Xing-feng |
| Affiliation: | TCM University of Hunan;Changsha;Hunan 410007 China |
| Abstract: | Objective To investigate the effects of drug-containing serum with Liuwei Di-huang micro-powder on proliferation and differentiation of preadipocytes in rats.Methods The preadipocytes were cultured,the proliferation change caused by the drug-containing serum was detected by MTT method,the expression of GPDH was determined by enzyme tis-sue chemical method,and the differentiation was determined by Oil Red O staining.Results The serum could stimulate preadipocyte proliferation and accumulation;inhibit the inc... |
| Keywords: | Liuwei Dihuang decoction micro-powder drug-containing serum preadipocytes proliferation rats rehmannia cornus poria |
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