三黄地榆散对多重耐药铜绿假单胞菌的抑制作用研究

目的 探讨三黄地榆散对多重耐药铜绿假单胞菌的抑制效果,为其临床应用提供依据.方法 首先制备中药含药血清用于体外最小抑菌浓度(MIC)、最小杀菌浓度(MBC)测定.然后建立烫伤大鼠感染多重耐药铜绿假单胞菌模型,分别给予西药硫酸庆大霉素和中药三黄地榆散灌胃,正常组和模型组灌胃等容量的无菌生理盐水;在给药后的第3、7、10天检测血液中炎性细胞和炎性因子的水平.结果 体外抑菌实验显示,三黄地榆散含药血清与庆大霉素对多重耐药铜绿假单胞菌的MIC、MBC均为512μg/mL.体内实验结果显示,与模型组比较均能显著下调大鼠血清WBC、IL-1β、TNF-α的水平(P<0.05).结论 三黄地榆散能抑制多重耐药铜绿假单胞菌的生长,对多重耐药铜绿假单胞菌感染烫伤大鼠治疗具有较好的疗效,机制可能与调节IL-1β、TNF-α水平有关.

Study on Inhibitory Effects of Multidrug Resistant Pseudomonas aeruginosa by Sanhuang Diyu Powder

Objective To investigate inhibitory effects of muitidrug resistant Pseudomonas aeruginosa by Sanhuang Diyu powder and provide the basis for its clinical application. Methods TCM serum was used for antibacterial experiment in vitro of minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The scald model rats infected by muitidrug resistant Pseudomonas aeruginosa were given with gentamicin sulphate and Sanhuang Diyu powder by gavage, re-spectively. Both the normal group and the model group were given with same volume sterile normal saline by gavage. At the first 3, 7, 10 days after delivery, neutrophils and inflammatory factors were determined. Results The results of bacteriostasis experiment in vitro indicated that MIC and MBC of Sanhuang Diyu powder and gentamicin on muitidrug resistant Pseudomonas aeruginosa both were 512 μg/mL. The results of bacteriostasis experiment in vivo indicated that Sanhuang Diyu powder and gentamycin could significantly decrease the levels of serum WBC, IL-1β and TNF-α in model rats (P<0.05). Conclusion Sanhuang Diyu powder has inhibitory effect aganist the growth of muitidrug resistant Pseudomonas aeruginosa in rats, which maby related to its ability of regulating IL-1β and TNF-α.