HPLC法测定黄花石蒜不同部位的加兰他敏含量

目的测定黄花石蒜不同部位加兰他敏的含量。方法采用HPLC法。色谱柱为Agilent Eclipse XDB-C18柱（150 mm×4.6 mm,5μm）,流动相为0.1%磷酸-甲醇（94∶6）,流速为1.0 mL/min,检测波长为238 nm,柱温为30℃,进样量为20μL。结果加兰他敏在10.4-124μg/mL范围内线性关系良好（r=0.999 7）,加样回收率102.8%,RSD为5.2%。黄花石蒜的花中加兰他敏含量最高,根次之,鳞茎和茎中含量最少。结论黄花石蒜不同部位中加兰他敏含量存在差异。

Determination of galanthamine from different parts of Lycoris aurea herb with HPLC

Objective To determine the content of galanthamine from different parts of Lycoris aurea herb with HPLC. Method There were the agilent eclipse XDB-C18 column （150 min×4.6 mm, 5 μm）, the mobile phase 0.1% phosphoric acid-methanol （94：6） at the flow rate of 1.0 mL/min, the detection wavelength was 238 nm, the column temperature 30 ℃, and the injection volume 20 μL in HPLC method. Results The linear range of galanthamine amount was 10.4-124 μg/mL（r=0.999 7）; the average recovery of galanthamine was 100.8% with RSD of 6.8%. The highest percentage of galanthamine was in the flowers of Lycoris aurea herb, next in the roots and the lowest in the stem and bulb. Conclusion The contents of galanthamine in different parts of Lycoris aurea herb are different.