清热解毒化浊片对ETM兔肝组织CD14及TNF-α、IL-1表达的影响

目的：探讨清热解毒化浊片对内毒素血症（Endotoxemia，ETM）模型兔肝功能、肝组织CD14和血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）的影响。方法将21只新西兰大耳白兔随机分为正常组、模型组、清热解毒化浊片组（清化组），每组7只。将模型组及清化组兔沿耳缘静脉注射大肠杆菌内毒素（LPS，10μg/kg），于造模后2 h干预，灌胃后2 h心脏采血检测家兔的肝功能、TNF-α和IL-α的变化，采肝脏组织检测CD14及观察肝脏病理形态。结果（1）与模型组比较，清化组ALT、AST上升（P＜0.05），肝组织CD14表达及血清TNF-α、IL-1下降(P＜0.05)；（2）模型组肝细胞排列结构紊乱，有炎性细胞浸润，且出现空泡样改变，清化组及正常组改变不明显。结论清热解毒化浊片能下调ETM模型兔CDl4、TNF-α和IL-1的表达，具有多靶点、多效应的干预作用，推测其有阻断早期LPS诱导信号通路的作用。

Effect of Qingre Jiedu Huazhuo Tablets on Expression of CD14 in Liver Tissue,TNF-α and IL-1 of ETM Rabbits

Objective To investigate the effect of Qingre Jiedu Huazhuo (QJH) tablets on the expression of CD14, TNF-α and IL-1 in liver tissue of ETM rabbits. Methods The 21 New Zealand big-eared rabbits were randomly divided into normal group, model group, QJH tablets group, 7 in each group. The ear edge of model group and QJH group was given intravenous injection of 10 μg/kg endotoxin. After 2 hours of modeling, the rabbites were given intervention. Then, the liver function, TNF-α and IL-1 were tested. The CD14 in liver tissue was determined and the pathological morphology was observed. Results (1) Compared with the model group, the ALT and AST of QJH tablets group were increased (P<0.05). The CD14 expression in liver tissue and serum TNF-α and IL-1 were decreased (P<0.05). (2) The liver cell array structure of model group was disorder with inflammatory cells infiltration and a cavity change, but the changes in QJH tablets group and blank group were not obvious. Conclusion Qingre Jiedu Huazhuo tablets with multi-point target and munti-effects can decrease CDl4, TNF-α and IL-1 in ETN rabbits, suggesting the tablets have an effect on the signaling pathway induced by LPS in the early days.