黄瓜根化学成分研究

目的 研究黄瓜根的化学成分.方法 采用硅胶柱层析和Sephadex LH-20凝胶柱层析进行分离纯化,通过理化性质和波谱数据分析进行结构鉴定.结果 从黄瓜根的乙酸乙酯提取物中分离了11个化合物,分别为:豆甾-4,6,8(14),22-四烯-3-酮(Ⅰ)、dehydroeburicoic acid(Ⅱ)、α-燕甾醇-3-O-β-D-葡萄糖苷(Ⅲ)、乌苏酸(Ⅳ)、3β-Hydroxymultiflora-8-en- 17-oic acid(Ⅴ)、Indolyl-3-acetic acid methyl ester(Ⅵ)、1-H-3-吲哚羧酸甲酯(Ⅶ)、吲哚醛(Ⅷ)、邻羟基苄醇(Ⅸ)、异香草醛(Ⅹ)和对羟基苯甲醛(Ⅺ).结论 除化合物Ⅲ外,其余化合物均为首次从该植物中分离得到.

Study on chemical constituents of cucumber roots

Objective To study the chemical constituents in roots of cucumber.Methods The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography,their structures were elucidated by the analysis of spectral data and chemical properties.Results Eleven compounds were isolated from EtOAc extract and their structures were identified as stigmasta-4,6,8(14),22-tetraen-3-one(Ⅰ),dehydroeburicoic acid(Ⅱ),α-spinasterol-3-O-β-D-glucopyranoside(Ⅲ),ursolic acid(Ⅳ),3β-Hydroxymultiflora-8-en-17-oic acid(Ⅴ),Indolyl-3-acetic acid methyl ester(Ⅵ),methyl 1H-indole-3-carboxylate(Ⅶ),1H-Indole-3-aldehyde(Ⅷ),2-hydroxybenzyl alcohol(Ⅸ),isovanillin(Ⅹ) and p-hydroxybenzaldehyde(Ⅺ).Conclusion These compounds were isolated from roots of Cucumis sativus L.for the first time except for α-spinasterol-3-O-β-D-glucopyranoside.