| 电针对心肌梗死后抑郁大鼠模型海马神经元作用机制的研究 |
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| 引用本文： | 郭磊磊,胡随瑜,张春虎,王素娥.电针对心肌梗死后抑郁大鼠模型海马神经元作用机制的研究[J].湖南中医药大学学报,2009,29(2):64-67. |
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| 作者姓名： | 郭磊磊 胡随瑜 张春虎 王素娥 |
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| 作者单位： | 中南大学湘雅医院中西医结合研究所,国家中医药管理局中医肝藏象重点研究室,湖南,长沙,410008 |
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| 基金项目： | 国家自然科学基金杰出青年科学基金 |
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| 摘 要： | 目的观察电针对心肌梗死后抑郁大鼠模型海马神经元的影响，并探讨可能的作用机制。方法建立心梗后抑郁症大鼠模型．采用电针干预的方法．观察大鼠的体质量和行为学变化。用TUNEL法和免疫组化检测海马神经元细胞凋亡。Western—blotting检测P—CREB。结果心梗后抑郁大鼠体质量、敞箱实验得分、糖水消耗和纯水消耗显著减少。大鼠海马CA3区出现神经元细胞凋亡，P-CREB水平降低。电针干预3周后，与模型组比较，大鼠体质量增加。行为学指标改善，神经元细胞凋亡教减少，Bcl-2表达增加，Bax表达减少，二者比值增高，并P—CREB水平增加。差异均具有统计学意义（P〈0．05）。结论心梗后抑都与海马神经元细胞凋亡和P—CREB水平降低有关．电针刺激可能涉及某些信号通路激活磷酸化CREB，抑制凋亡基因，促进抗凋亡基因的产生。
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| 关 键 词： | 电针 心肌梗死 抑郁症 动物模型 大鼠 海马神经元 |
Mechanism of electro-acupuncture on hippocampus neurons after myocardial infarction in rats with depression |
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| GUO Lei-lei,HU Sui-yu,ZHANG Chun-hu,WANG Su-e.Mechanism of electro-acupuncture on hippocampus neurons after myocardial infarction in rats with depression[J].Journal of Traditional Chinese Medicine University of Hunan,2009,29(2):64-67. |
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| Authors: | GUO Lei-lei HU Sui-yu ZHANG Chun-hu WANG Su-e |
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| Institution: | Institute of Combined Traditional Chinese and Western Medicine;Xiangya Hospital;Central South University;Changsha;Hunan 410008;China |
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| Abstract: | Objective To observe the effect of electro-acupuncture(EA) on the hippocam-pus neurons in rats with depression after myocardial infarction(MI),and investigate the possible mechanism on it.Methods The animal model of depression after MI was established and treat-ed with EA.All rats were tested on weight and ethology.The neurons apoptosis of hippocampus were examined with TUNEL and immunohistochemicstry.The level of p-CREB was determined with Western-blotting.Results The weight of rats,score of open-field tes... |
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| Keywords: | electro-acupuncture myocardial infarction depression animal model rats hippocampus neurons |
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