人参总皂甙诱导人骨髓基质细胞表达GM-CSF的实验研究

目的：研究人参总皂甙(TSPG)对人骨髓基质细胞表达GM—CSF的影响及其与人粒单系血细胞发生的关系。方法：采用造血祖细胞与骨髓基质细胞体外培养、造血生长因子生物学活性检测、免疫细胞化学和核酸分子原位杂交等技术。结果：TSPG能显著刺激粒单系造血祖细胞(CFU—GM)增殖；经TSPG诱导制备的骨髓基质细胞条件培养液富含GM—CSF样活性；TSPG能显著促进骨髓基质细胞的GM—CSF蛋白和mRNA表达。结论：TSPG可能通过直接和/或间接途径促进造血诱导微环境中的骨髓基质细胞合成和分泌GM—CSF，进而促进CFU—GM的增殖分化。

Experimental study on expressing GM-CSF in human bone marrow stromal cells by total saponins of Panax ginseng (TSPG)

Objective: To study the relationship between effect of total saponins of Panax ginseng(TSPG) on expression of GM-CSF in human bone marrow stromal cells and modulation of human granulocytopoiesis and monocytopoiesis.Methods:The techniques of culture of hematopoietic progenitor cells and human bone marrow stromal cells in vitro,immunocytochemistry, nucleic acid in situ hybridization were used.Results:TSPG could markedly promote the colony formation of CFU-GM; human bone marrow stromal cells conditioned media prepared with TSPG could significantly enhance the proliferation and differentiation of CFU-GM,and expression of GM-CSF in protein and mRNA level in human bone marrow stromal cells induced by TSPG were much higher than those of the control group.Conclusion:TSPG may directly and/or indirectly promote stromal cells in hematopoietic inductive microenvironment to produce GM-CSF, which further prowote the proliferation and differentiation of human CFU-GM.