VEGF-C反义寡核苷酸对卵巢癌细胞SKOV3的黏附、侵袭能力的影响

目的:探讨VEGF-C反义寡核苷酸(VEGF-C,ASODN)对人卵巢癌细胞SKOV3黏附、侵袭能力的影响.方法:以VEGF-C基因编码区为靶点合成反义寡核苷酸,脂质体介导转染.MTT法检测卵巢癌细胞与细胞外基质黏附能力;利用transwell小室,检测卵巢癌细胞侵袭人工基底膜的能力;免疫组化法检测细胞中VEGF-C蛋白表达,western blot检测细胞中MMP-2蛋白表达.结果:与空白对照组及正义序列转染组相比,ASODN组对细胞外基质黏附率明显降低(P＜0.01),穿透重组基质膜数目明显下降(P＜0.01),细胞中VEGF-C、MMP-2蛋白表达明显下降(P＜0.01).结论:VEGF-C ASODN可明显抑制卵巢癌细胞体外实验中的黏附和侵袭能力,下调MMP-2的表达可能是其作用途径.

Effect of VEGF-C ASODN on adhesion and invasion of ovary cancer cells

Objective:To investigate the effect and the mechanism of VEGF-C antisense oligodeoxynucleotide(ASODN) on the adhesion and invasion of human ovary cancer cell line SKOV3 in vitro.Methods:The ASODN sequence targeting the coding region of VEGF-C was synthesized and introduced into ovary cancer cells by liposomes.The expression of VEGF-C protein was detected by inmmnohistochemistry;the adhesion of SKOV3 cells to artifical basement membrane Matrigel was measured by MTT assay.The invasion was evaluated by transwell chambers attached with polycarbonate filters and reconstituted basement membrane(Matrigel).The expression of MMP-2 protein was measured by western blot.Result:In ASODN group,the adhesive rate and the invasive cell numbers were decreased markedly(P<0.01)compared with the control and sense oligodeoxynucleotide(SODN)group.The VEGF-C protein level was significantly decreased(P<0.01).The result of western blot showed a down-regulated MMP-2 expression(P<0.01).Conclusion:In vitro,the abilities of adhesion and invasion of ovary cancer cells can be suppressed by VEGF-C ASODN.Down-regulating the expressin of MMP-2 may be the mechanism.