首页 | 本学科首页   官方微博 | 高级检索  
检索        

缺口连接酶链反应检测沙眼衣原体DNA的实验研究
引用本文:韦红,吴仕孝,杨军,余加林,伍金林,刘官信.缺口连接酶链反应检测沙眼衣原体DNA的实验研究[J].重庆医科大学学报,2004,29(1):8-10.
作者姓名:韦红  吴仕孝  杨军  余加林  伍金林  刘官信
作者单位:重庆医科大学儿科学院新生儿科,重庆,400014
摘    要:目的:建立缺口连接酶链反应方法检测沙眼衣原体.方法:根据编码CT主要外膜蛋白的基因(ompl)的稳定区设计2对互补的寡核苷酸探针.采用G-LCR和常规PCR扩增CT标准株DNA,并对二者检测CT的敏感性进行比较.结果:对5种CT标准株进行G-LCR扩增,均出现54bp长度的DNA片段.敏感性实验可检测出2个CT原体,而PCR可检测出20EBs.G-LCR较PCR敏感10倍;在特异性方面,不扩增肺炎衣愿体及其他细菌DNA,具有很高的特异性.结论:G-LCR用于检测沙眼衣原体特异、敏感、快速、准确,可为CT感染的临床诊断提供依据.

关 键 词:沙眼衣原体  连接酶链反应  聚合酶链反应
文章编号:0253-3626(2004)01-0008-03

Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)
WEI Hong,et al.Detection of Chlamydia trachomatis DNA by gap ligase chain reaction (G-LCR)[J].Journal of Chongqing Medical University,2004,29(1):8-10.
Authors:WEI Hong  
Abstract:Objective:To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:Using G-LCR,DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:G-LCR is sensitive,rapid and specific for detection of Chlamydia trachomatis.
Keywords:Chlamydia trachomatis  Ligase chain reaction  Polymerase chain reaction
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号