Bcl-w蛋白在原发性胆囊癌中的表达及意义

目的 研究Ｂｃｌ－ｗ蛋白在原发性胆囊癌（ｐｒｉｍａｒｙ ｇａｌｌｂｌａｄｄｅｒ ｃａｒｃｉｎｏｍａ，ＰＧＢＣ）中的表达及其意义。方法 以特异性羊抗免Ｂｃｌ－ｗ抗体，ＤＡＫＯ Ｅｎｖｉｓｉｏｎ＾ＴＭ系统（ＬＤＰ法）免疫组织化学方法检测石蜡我埋的ＰＧＢＣ标本中Ｂｃｌ－ｗ蛋白的表达。结果 ４７例ＰＧＢＣ中Ｂｃｌ－ｗ阳性２８例（６０％）；１５例胆囊腺肌瘤样增生（ＧＢＡＤＨ）Ｂｃｌ－ｗ阳性１４例（９３％）；

Expression and its significance of antiapoptosis protein Bcl-w in primary gallbladder carcinomas

AIM To study the protein expression of Bcl w in primary gallbladder carcinoma (PGBC) and its clinical signifi cance. METHODS Protein expression levels of Bcl w were determined by immunohistochemistry with rabbit antihuman Bcl w antibody and Envision TM system. RESULTS Bcl w was detectable in 60% of PGBC (28 of 47) and detectable in 93% of the gallbladder adenomymatous hyperlasia (GBADH) (14 of 15); Bcl w levels were significantly higher in GBADH than in PGBC ( P <0.05). Bcl w levels were statistically higher in patients whose tumor sizes were no more than 2 cm, male and senior to 55 years old than those whose tumor size was less than 2 cm, female and junior to 55 years old ( P <0.05, P <0.05, P <0.01, respectively), Bcl w expression levels were not associated with any of Nevin's stages. CONCLUSION Antiapoptotic protein Bcl w partially participates in apoptosis regulation in PGBC, and its expression levels are correlated with clinical situation in PGBC.