流式细胞仪测定成人急性白血病患者DNA含量的临床意义

为探讨急性白血病患者骨髓细胞的周期分布，非整倍体，及其与临床疗效的关系。作者应用流式细胞仪测定了５７例ＡＬ患者的骨髓细胞ＤＮＡ含量。不同类型ＡＬ患者骨髓细胞Ｓ％均明显低于正常对照和缓解后患者。急性早幼粒细胞白血病Ｓ％最低；ＡＬ患者骨髓细胞ＤＮＡ非整倍体检出率２９．８％，４例完全缓解的患者仍检出ＤＮＡ非整倍体细胞，随化疗进展而消失。

Clinical significance of DNA content assay in adult acute leukemia by flow cytometry

Objective: To investigate the marrow cell circle distribution, aneuploid, and its relation with therapeutic effects in adult acute leukemia (AL) patients. Method: Bone marrow cellular DNA content was assessed in 57 patients with acute leukemia by flow cytometry, the relationship between S phase percent and response to antileukemic effect of induction chemotherapy was evaluated. Results: Forty six untreated patients had a smaller S phase compartment than patients with complete remission and normal control. Acute promyelocytic leukemia (M 3) had the smallest S phase compartment. The proportion of patients with DNA aneuploidy in acute leukemia was 29.8%. Abnormal DNA content cells in four patients after complete remission were still determined. The relationship between S phase compartment and blast reduction index (BRI) showed that high S phase group was more sensitive than low S phase group in response to chemotherapy, but complete remission patients with high S phase compartment were apt to relapse. Complete remission rate of both groups was of no difference. Conclusion: Analysis of marrow cell DNA in AL patients by FCM can provide the crucial reference data for treatment.