| 荔枝核黄酮类化合物对HepG2.2.15细胞系HBsAg与HBeAg表达及HBV-DNA含量的影响 |
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| 引用本文： | 徐庆,宋芸娟,陈全斌,义祥辉.荔枝核黄酮类化合物对HepG2.2.15细胞系HBsAg与HBeAg表达及HBV-DNA含量的影响[J].第四军医大学学报,2004,25(20):1862-1866. |
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| 作者姓名： | 徐庆 宋芸娟 陈全斌 义祥辉 |
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| 作者单位： | 1. 桂林医学院药理学教研室,广西,桂林,541004
2. 广西师范大学化学化工学院,广西,桂林,541004 |
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| 基金项目： | 广西自然科学基金，广西科学研究与技术开发计划项目 |
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| 摘 要： | 目的:研究荔枝核提取物-黄酮类化合物(IL)对乙肝病毒的抑制作用.方法:应用光密度测定法和细胞培养及定量PCR技术研究荔枝核提取物IL对HepG2.2.15细胞系HBsAg与HBeAg表达及HBV-DNA含量的影响. 结果: 96孔板试验: 实验第3, 6日, IL对HBsAg和HBeAg表达均有明显的抑制作用(与对照组比较P<0.01). 24孔板试验: 实验第3, 6, 9日IL对HBsAg表达有明显的抑制作用(与对照组比较P<0.01);实验第6, 9日IL对HBeAg表达有明显的抑制作用(与对照组比较P<0.01);同时采用定量PCR方法检测,IL (400 mg/L )可使培养基中的HBV-DNA转阴. 结论: IL体外实验(培养细胞)有较强的抗乙肝病毒作用.
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| 关 键 词： | 荔枝核 黄酮类 HepG2.2.15细胞 肝炎表面抗原 乙型 肝炎e抗原 乙型 肝炎病毒 乙型 |
| 文章编号： | 1000-2790(2004)20-1862-05 |
| 修稿时间： | 2004年7月29日 |
Effects of flavonoids extracted from core of Litchi Chinensis Sonn on HBsAg and HBeAg expression and HBV-DNA content in HepG2.2.15 cells |
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| XU Qing ,SONG Yun-Juan ,CHEN Quan-Bin ,YI Xiang-Hui.Effects of flavonoids extracted from core of Litchi Chinensis Sonn on HBsAg and HBeAg expression and HBV-DNA content in HepG2.2.15 cells[J].Journal of the Fourth Military Medical University,2004,25(20):1862-1866. |
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| Authors: | XU Qing SONG Yun-Juan CHEN Quan-Bin YI Xiang-Hui |
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| Institution: | XU Qing 1,SONG Yun-Juan 2,CHEN Quan-Bin 2,YI Xiang-Hui 2 1Department of Pharmacology,Guilin Medical College,2School of Chemistry & Chemical Engineering,Guangxi Normal University,Guilin 541004,China |
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| Abstract: | AIM: To study the anti-HBV action of flavonoids ( IL ) extracted from the core of Litchi Chinensis Sonn.METHODS: Optical density mensuration, cell culture and FQ - PCR technique were used to study the inhibitory effects of IL extracted from the core of Litchi Chinensis Sonn on the expressions of HBsAg and HBeAg and the content of HBV-DNA in HepG2.2.15 cells.RESULTS: In 96-hole plate test, IL (800, 400, 200, 100 mg/L)had obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2.2.15 cells on the third and sixth experiment days, compared with that of the control group (P<0.01). In 24-hole plate test, IL (400, 200, 100, 50 mg/L) had obvious inhibitory effects on the expression of HBsAg on the third, sixth and ninth experiment days and on the expression of HBeAg on sixth and ninth experiment days compared with those of the control group (P<0.01). FQ-PCR showed that IL (400 mg/L) turned HBV-DNA in HepG2.2.15 cells medium negative. CONCLUSION: IL has strong anti-HBV action in vitro. |
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| Keywords: | DNA |
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