胆囊结石需氧菌和厌氧菌分子生物学研究及意义

研究胆囊结石细胞脱氧核糖核酸，提取供细胞在胆囊结石核心形成作用中分子生物学证据。方法：采用聚合酶锭反应对７０例胆结石中５种需氧菌和４种厌氧菌ＤＮＡ进行检测。结果总的检测出细菌ＤＮＡ６０例，其中需氧菌ＤＮＡ存在４３例，厌氧菌ＤＮＡ存在１７例。

A study on molecular biology of aerobic and anaerobic bacteria in disease with gallstone patients

Aim: To study deoxyribonucleic acid (DNA) ofaerobic and anaerobic bacteria in gallstones and provideevidence of molecular biology by bacteria value in gallstonesformation nuclear. Methods: DNAs of five aerobic and fouranaerobic bacteria were tested with polymerase chainreaction (PCR) in 70 gallstones patients. Results: Measured allDNAs of bacteria were 60 patients (85. 71%), which DNAsof aerobic and anaerobic bacteria were 43 patients (61.43%) and 17 patients (24. 29%), respectively. DoubleClasses coexistent bacteria DNAs were 11 patients (15.71%). DNAs of Helicobacter Pylori were tested in 15gallstones patients at first. Conclusion: Existent bacteria ofgallstones were demonstrated in molecular biology level.Bacteria may be important factor to form gallstone nuclear.