Ras-MAPK信号转导系统在哮喘气道重塑中的作用

目的 探讨Ras-MAPK信号转导系统在哮喘气道重塑中的作用。方法 以卵蛋白诱导并激发的豚鼠哮喘模型为研究对象，选择末次诱喘后即刻至240min间数个时间点，采用Dot-blot分子杂交法检测气道ras mRNA水平。免疫组化ABC研究气道Ras和MAPK蛋白的表达情况。结果 与正常对照组相比，哮喘组豚鼠气道上皮水肿、脱落，平滑肌层及基底膜显著增厚。诱喘后ras mRNA的表达水平明显上调，30min达高峰并维持至120min。各级气道的上皮和平滑肌细胞Ras表达量显著增加，而MAPK只在气道平滑肌层呈现高表达现象。结论 Ras-MAPK信号转导系统在哮喘气道重塑早期发挥重要作用，阻断这一途径有望延缓重塑的病理过程。

Role of Ras-MAPK signal transduction pathway in airway remodeling of asthma

AIM To investigate the role of Ras MAPK signal transduction pathway in the remodeling of airway in asthma. METHODS The asthma models in guinea pigs, which were induced and provoked by ovoglubulin, were used in this study, and then the level of ras mRNA in airway were examined by Dot blot molecular hybridization at several pointsafter provokation (0, 30, 60, 120, 240 min). Immunohistochemical techniques were used to observe the expressions of Ras and MAPK, respectively. RESULTS Compared with the normal group, asthma animal airway showed edema and drop of epithelium cells, thickening of smooth muscle layer and base membrane. And the level of ras mRNA increased obviously, reaching the peak 30 min and maintaining to 120 min after the provocation. The expression of Ras was also enhanced in epithlium and smooth muscle layers of airway, but MAPK increased only in smooth muscle cells. CONCLUSION Ras MAPK signal transduction pathway plays an important role in airway remodeling in asthma at early age, thus blocking the pathological process may be a potential treatment strategy.