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青蒿素对小鼠Lewis肺癌移植瘤生长和淋巴管生成的影响
引用本文:郭燕,王俊,章必成,陈正堂,高建飞,赵勇.青蒿素对小鼠Lewis肺癌移植瘤生长和淋巴管生成的影响[J].第四军医大学学报,2007,28(4):357-360.
作者姓名:郭燕  王俊  章必成  陈正堂  高建飞  赵勇
作者单位:1. 第三军医大学新桥医院全军肿瘤研究所,重庆,400037
2. 广州军区武汉总医院肿瘤科,湖北,武汉,430070
摘    要:目的:探讨抗疟药青蒿素对Lewis肺癌生长和淋巴管生成的作用.方法:通过接种(sc)Lewis肺癌细胞建立C57BL/6小鼠Lewis肺癌移植瘤模型,灌胃给予50 mg/kg的青蒿素,1次/2 d,连续给药2 wk;游标卡尺观察给药后肿瘤体积变化. 接种后第30 d处死动物,收集肿瘤组织和双肺组织,并称质量. 免疫组化检测瘤内和瘤周VEGFR-3和LYVE-1表达,计数LYVE-1阳性淋巴管并计算微淋巴管密度,HE染色肺组织计数肺转移结节数,观察荷瘤小鼠的存活率.结果:与对照组比较,青蒿素组瘤内LMVD,肺湿重,肺转移发生率和肺转移结节数均较低(P<0.05);青蒿素治疗组与对照组移植瘤生长没有明显差异(P>0.05);青蒿素治疗组小鼠存活率与对照组比较明显增加(P<0.01).结论:青蒿素可有效抑制Lewis肺癌淋巴管生成和肺转移,并提高小鼠存活率.

关 键 词:青蒿素    Lewis肺  淋巴管生成  血管内皮生长因子-C
文章编号:1000-2790(2007)04-0357-04
修稿时间:2006-10-09

Effect of artemisinin on metastatic tumor growth and lymphangiogenesis in mice bearing Lewis lung carcinoma
GUO Yan,WANG Jun,ZHANG Bi-Cheng,CHEN Zheng-Tang,GAO Jian-Fei,ZHAO Yong.Effect of artemisinin on metastatic tumor growth and lymphangiogenesis in mice bearing Lewis lung carcinoma[J].Journal of the Fourth Military Medical University,2007,28(4):357-360.
Authors:GUO Yan  WANG Jun  ZHANG Bi-Cheng  CHEN Zheng-Tang  GAO Jian-Fei  ZHAO Yong
Institution:1Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China; 2Department of Oncology, Wuhan General Hospital, Guangzhou Military Area Command, Wuhan 430070, China
Abstract:AIM: To explore the effect of antimalarial artemisinin on Lewis lung carcimoma (LLC) growth and tumor lymphangiogenesis. METHODS: The models of LLC in C57BL/6 mice were established via subcutaneous injection of mouse LLC cells. After mice were orally administered with artemisinin at 50 mg/kg body weight, once 2 d for 2 weeks, tumors were measured with calipers twice weekly. Mice were sacrificed 30 d following tumor cell injection, and Lewis tumors and lungs were harvested and weighted. Lewis tumors and lungs were processed for immunohistochemical analysis for lymphangiogenesis using VEGFR-3 and LYVE-1 staining or for micrometastasis using HE staining. Lymphatic microvessel density (LMVD) was evaluated by LYVE-1 positive lymphatic vessel count. Furthermore, mice were monitored for survival studies. RESULTS: LMVD, wet weight of lungs and number of lung metastasis in artemisinin-treated group were significantly lower as compared with control group (P<0.05). However there was not statistically significant difference in tumor growth between artemisinin-treated mice and control mice (P>0.05). In addition, artemisinin-treated mice lived significantly longer than the control mice did (P<0.01). CONCLUSION: Antimalarial artemisinin effectively inhibits the lymphangiogenesis and lung metastasis, and enhances the survival of mice bearing LLC.
Keywords:artemisinin  carcinoma  Lewis lung  lymphangiogenesis  vascular endothelial grouth factorc
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