细胞因子对HCV基因疫苗诱生免疫反应的增强作用

目的 探讨细胞因子对丙型肝炎病毒（ＨＣＶ）核心（Ｃ）基因疫苗诱生免疫应答强度的增强作用。方法 将包含ＨＣＶ－Ｃ蛋白的基因片段插入真核表达载体ｐｃＤＮＡ３中，构建重组质料ｐｃＤＮＡＨＣＶ－Ｃ，将此重组质粒单独或与包含鼠ＩＬ－２或ＩＬ－１２基因的表达质粒共免疫ＢＡＬＢ／ｃ小鼠，ＥＬＩＳＡ法检测ＨＣＶ Ｃ特异性抗体产生情况，以ｐｃＤＮＡＨＣＶ－Ｃ转梁小鼠ＳＰ２／０细胞，表达ＨＣＶ Ｃ抗原为靶细胞，进行

Cytokine enhances immune responses to DNA vaccine of HCV

AIM To investigate the enhancing effect of IL-2 or IL-12 on immune responses of BALB/c mice to HCV C gene. METHODS BALB/c mice were immunized with a recombinant plasmid containing HCV-C gene (pcDNAHCV-C) alone or together with a constructs encoding IL-2 or IL-12(pIL-2 or pIL-12). Anti-HCV-C specific antibody in the sera was measured with ELISA. Spleen cells from experimental and control mice were extracted. The mouse myloma SP2/0 cells expressing HCV C antigen served as target cells. CTL activity was measured by standard 4-h 51 Cr release assays. RESULTS Anti-HCV-C antibody OD value from 1.21 of pcDNAHCV-C alone increased to 2.09 and 1.73 of coimmunized with pIL-2 or pIL-12 respectively. CTL activity to HCV core protein was enhanced substantially after coimmunization with the IL-12 expression plasmid. In contrast, the improvement of HCV core-specific CTL activity with an IL-2-producing construct coimmunization was not obvious, which maybe resulted from the low-level expression of IL-2 vector-pDOR. CONCLUSION DNA vaccine of HCV combined with IL-12 expressive plasmid can get better therapeutic effects, and it might represent an approach to the gene therapy against HCV infection.