| 人脂肪干细胞分泌因子对HaCaT细胞的生物学作用 |
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| 引用本文： | 折涛,刘佳琦,张军,张万福,蔡维霞,白哓智,胡大海.人脂肪干细胞分泌因子对HaCaT细胞的生物学作用[J].第四军医大学学报,2009(13):1206-1208. |
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| 作者姓名： | 折涛 刘佳琦 张军 张万福 蔡维霞 白哓智 胡大海 |
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| 作者单位： | 第四军医大学西京医院全军烧伤中心; |
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| 基金项目： | 国家自然科学基金(30772249) |
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| 摘 要： | 目的：分离培养脂肪干细胞（ADSCs）以探讨其分泌因子对HaCaT细胞的生物学作用.方法：从人腹部脂肪组织中分离并培养ADSCs3d后,ELISA法测定ADSCs分泌血管内皮生长因子（VEGF）,肝细胞生长因子（HGF）含量;收集ADSCs培养液（ADSC-CM）作用于HaCaT细胞株.采用MTT法测定HaCaT细胞的增殖;AnexinV/PI双染色法测定HaCaT细胞的凋亡情况;体外细胞划痕法测定其迁移能力.结果：培养获得ADSCs细胞3d后,培养液中VEGF、HGF含量分别为（287±47）,（577±85）ng/L.MTT实验30%ADSC-CM组、50%ADSC-CM组的A490nm值分别高于对照组,差异具有统计学意义（P〈0.05或P〈0.01）.实验组HaCaT细胞凋亡率明显低于对照组（P〈0.05）.与对照组相比较,实验组HaCaT细胞迁移距离36,48h时间点显著增大（P〈0.05或P〈0.01）.结论：ADSCs能分泌细胞因子且对HaCaT细胞具有促增殖、迁移和抑制凋亡作用.
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| 关 键 词： | 脂肪干细胞 HaCaT细胞 增殖 迁移 凋亡 |
Effects of conditioned medium of human ADSCs on HaCaT cells |
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| SHE Tao,LIU Jia-Qi,ZHANG Jun,ZHANG Wan-Fu,CAI Wei-Xia,BAI Xiao-Zhi,HU Da-HaiPLA Burns Center,Xijing Hospital,Fourth Military Medical University,Xi'an ,China.Effects of conditioned medium of human ADSCs on HaCaT cells[J].Journal of the Fourth Military Medical University,2009(13):1206-1208. |
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| Authors: | SHE Tao LIU Jia-Qi ZHANG Jun ZHANG Wan-Fu CAI Wei-Xia BAI Xiao-Zhi HU Da-HaiPLA Burns Center Xijing Hospital Fourth Military Medical University Xi'an China |
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| Institution: | SHE Tao,LIU Jia-Qi,ZHANG Jun,ZHANG Wan-Fu,CAI Wei-Xia,BAI Xiao-Zhi,HU Da-HaiPLA Burns Center,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China |
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| Abstract: | AIM:To isolate and culture adipose-derived stem cells(ADSCs) and to study the effects of cytokines secreted by ADSCs on HaCaT cells. METHODS: ADSCs were isolated from human adipose tissues and cultured for 3 d. ELISA was used to detect the secretion level of VEGF and HGF. The conditioned medium of ADSCs(ADSC-CM) was then used to culture HaCaT cells. MTT method was used to determine the proliferation and Annexin V/PI was used to examine the apoptosis rate of HaCaT cells. The cell migration was measured by in... |
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| Keywords: | adipose-derived stem cells HaCaT cells proliferation migration apoptosis |
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