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胰岛素作用对胰岛β细胞磷脂酶Cγ1表达的影响
引用本文:李广文,焦凯.胰岛素作用对胰岛β细胞磷脂酶Cγ1表达的影响[J].第四军医大学学报,2009(19):1944-1946.
作者姓名:李广文  焦凯
作者单位:第四军医大学唐都医院内分泌科;
摘    要:目的:观察胰岛素对大鼠胰岛β细胞内磷脂酶Cγ1(PLCγ1)表达的影响.方法:大鼠胰岛分离纯化.通过HE染色观察纯化胰岛的形态特征,采用免疫组织化学方法并结合激光扫描共聚焦显微镜和图像分析技术对胰岛β细胞表达不同时间(0,30,60,120,240min)内的PLCγ1进行半定量分析.结果:PLCγ1在胰腺的内外分泌部均有表达.与0min比较,胰岛素作用30,60min时胰岛β细胞表达的PLCγ1荧光强度有明显降低(P〈0.05);而在240min时PLCγ1荧光强度与0min比较无明显差别.结论:胰岛素作用60min内可使PLCγ1表达下调;胰岛素-PLCγ1途径参与了胰岛素的信息传递.

关 键 词:磷脂酶Cγ1  胰岛素  胰岛  β细胞

Effect of insulin function on expression of phospholipaseCγ1 in β cells
LI Guang-Wen,JIAO Kai.Effect of insulin function on expression of phospholipaseCγ1 in β cells[J].Journal of the Fourth Military Medical University,2009(19):1944-1946.
Authors:LI Guang-Wen  JIAO Kai
Institution:LI Guang-Wen,JIAO Kai Department of Endocrinology,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China
Abstract:AIM:To observe the effect of insulin on expression of phospholipaseCγ1 in β cells of rat islets.METHODS:Rat islets were isolated and purified.The morphological features were shown with hematoxylin-eosin(HE)dyeing.The expression of PLCγ1 in different times(0,30,60,120 and 240 min)was semiquantitatively analyzed by method of immunocytochemistry combined with laser scanning confocal microscopy and image analyzed technology.RESULTS:PLCγ1 was expressed in the endocrine and exocrine pancreas.The expression of PLCγ1 in β cells for 30 and 60 min with insulin incubation was obviously decreased compared with that at 0 min(P〈0.05).And there was no obvious change at 240 min compared with insulin incubation.CONCLUSION:The expression of PLCγ1 was decreased at 60 min by the inducing of insulin.Insulin-PLCγ1 was concerned with the insulin information transformation.
Keywords:phospholipaseCγ1  insulin  pancreatic islets  β cells
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