| 抗c—erbB—2 ribozyme的计算机设计 |
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| 引用本文： | 毕锋,张学庸.抗c—erbB—2 ribozyme的计算机设计[J].第四军医大学学报,1997,18(1):6. |
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| 作者姓名： | 毕锋 张学庸 |
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| 作者单位： | 第四军医大学西京医院全军消化疾病研究所!西安710033(毕锋,张学庸,樊代明),第四军医大学生物化学及分子生物学教研室(惠宏襄,王成济) |
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| 基金项目： | 国家自然科学基金资助课题No.39500066 |
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| 摘 要： | 设计能特异性切割人癌基因ｃ－ｅｒｂＢ－２ｍＲＮＡ的核酶。概据Ｓｙｍｏｎｘ的“猛士锤头结构”,以人癌基因ｃ－ｅｒｂＢ－２ｍＲＮＡ为靶ＲＮＡ,用计算机对其可能为核酶切割的位点进行分析以寻找最佳切点。对底物及核酶的二级结构进行预测。
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| 关 键 词： | c－erbB－2 ribozyme 计算机设计 基因疗法 肿瘤 |
Computer design of anti-c-erbB-2 ribozymes |
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| Bi Feng,Zhang Xueyang,Fan Daiming,Hut Hangxiang,Wang ChengjiInstitute of Digestive Disease of PLA,Fourth Military Medical University,Xi'an.Computer design of anti-c-erbB-2 ribozymes[J].Journal of the Fourth Military Medical University,1997,18(1):6. |
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| Authors: | Bi Feng Zhang Xueyang Fan Daiming Hut Hangxiang Wang ChengjiInstitute of Digestive Disease of PLA Fourth Military Medical University Xi'an |
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| Institution: | Bi Feng,Zhang Xueyang,Fan Daiming,Hut Hangxiang,Wang ChengjiInstitute of Digestive Disease of PLA,Fourth Military Medical University,Xi'an 710033 Department of Biochemistry and Molecular Biology,Fourth Military Medical University |
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| Abstract: | Objective:To design the ribozymes which may cleave the mRNA of human c-erbB-2 oncogene. Methods: The c-erbB-2 mRNA was taken as the target RNA. Ribozymes were designed according to the "hammerhead structure" described by Symons. Computer was used to analyze the possible secondary cleavage sites on c-erbB-2 mRNA and to predict the secondary structures of substrate and ribozymes. Results: The secondary structures of c-erbB-2 mRNA from nt 2428 to nt 2776 and nt 2820 to nt 3004 were relatively stable. The two regions were of important biological function and were the ideal attacking regions for ribozymes. Three ribozymes targeting the nt 2438, nt 2477 and nt 2751 on the c-erbB-2 mRNA were designed and were named RZ1, RZ2 and RZ3 respectively. The hammerhead structures composed of ribozymes and targeting mRNA could be formed by computer imitation. No analogous sequence of substrate which combined with ribozyme was found in the mRNA of other genes in known human Gen Bank through computer probe. Conclusion: Not all GUX or CUX could be taken as the cleavage site of ribozyme. The nt 2438, nt 2477 and nt 2751 on the c-erbB-2 mRNA are the most ideal attacking sites for ribozymes. |
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| Keywords: | c-erbB-2 ribozyme computer design |
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