| 土贝母提取物——结晶E对HL-60细胞的诱导分化作用 |
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| 引用本文： | 白月辉,黄昌亮.土贝母提取物——结晶E对HL-60细胞的诱导分化作用[J].第四军医大学学报,1992(4). |
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| 作者姓名： | 白月辉 黄昌亮 |
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| 作者单位： | 唐都医院血液病科
(白月辉)，唐都医院血液病科(黄昌亮) |
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| 摘 要： | 作者以短期液体培养方法,通过细胞活力测定、形态分类、酵母多糖吞噬试验、NBT还原试验及非特异性酯酶染色等指标,观察了土贝母提取物——结晶E(ST-E)对人早幼粒白血病细胞(HL-60)的诱导分化作用.结果发现ST-E能诱导HL-60细胞向类似成熟中性粒细胞方向分化,细胞形态变化显著,核浆比例变小,核呈肾形或杆状,核染色质浓缩增粗,核仁消失,胞浆嗜天青颗粒消失,还原NBT和吞噬酵母多糖能力显著增强,非特异性酯酶染色阴性,提示ST-E可能是一种新的抗白血病有效药物.
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| 关 键 词： | 上贝母提取物——结晶E 医学 中国传统 HL-60细胞诱导分化 |
Induction of differentiation of HL-60 cell by extracting bolbostemma paniculatum franquet-crystal-E |
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| BAI Yue-Hui and HUANG Chang-Liang.Induction of differentiation of HL-60 cell by extracting bolbostemma paniculatum franquet-crystal-E[J].Journal of the Fourth Military Medical University,1992(4). |
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| Authors: | BAI Yue-Hui and HUANG Chang-Liang |
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| Institution: | BAI Yue-Hui and HUANG Chang-Liang Department of Haematology,Tangdu Hospital |
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| Abstract: | The extract of bolbostemma paniculatum (Maxion) franquet-crystal-E (ST-E) induced differentiation ofhuman promyelocytic leukemia cells (HL-60) into mature-like granulocytes in liquid culture. The cells became closely resembled to normal metamyelocytes or banded and segmented neutrophils, with increased amount cytoplasm and loss of azurophilic granule. ST-E caused significant increase in their ability to phagocytize zymosan particles and to reduce nitroblue tetrazolium (NBT). The cell's staining for a-nephthyl AS-D acetate (nonspecific) esterase was uniformly negative. The above findings suggest that ST-E may be an effective antileukemic drug. |
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| Keywords: | extract of bolbostemma paniculatum franquet-crystal-E medicine Chinese traditional HL-60 cell cell differentiation |
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