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缺血后处理对缺血再灌注大鼠心肌梗死和心肌细胞凋亡的作用
引用本文:李源,陶凌,臧益民,高峰.缺血后处理对缺血再灌注大鼠心肌梗死和心肌细胞凋亡的作用[J].第四军医大学学报,2002,23(18):1690-1693.
作者姓名:李源  陶凌  臧益民  高峰
作者单位:1. 第四军医大学西京医院老年病科,陕西,西安,710033
2. 第四军医大学西京医院,基础部生理学教研室,陕西,西安,710033
基金项目:国家自然科学基金,Fund for Returned Scholar of PLA,39970302,98H019,,
摘    要:目的观察在体情况下缺血后处理(IPC)对兔心肌梗死范围及缺血心肌细胞凋亡的影响,并与缺血预处理(IP)心脏保护作用比较. 方法采用兔心肌缺血/再灌注模型,在缺血后、再灌注前多次短暂再灌/停灌处理. 以Even's blue-TTC法检测心肌梗死范围,TUNEL方法检测缺血心肌细胞凋亡. 结果与对照组相比,缺血后处理明显减小心肌梗死范围(12.5±5.4% vs对照组26.7±6.7%, P<0.01),缺血区心肌凋亡指数明显下降(14.6±7.4 vs对照组30.4±12.3, P<0.05). 结论对于已缺血心肌,再灌前予多次短暂复灌、停灌处理具有与IP类似缩小心梗范围作用,IPC对缺血心肌的保护效应可能与其抑制缺血心肌细胞凋亡有关.

关 键 词:缺血后处理  缺血再灌注  大鼠  心肌梗死  心肌细胞  凋亡

Effects of ischemic postconditioning on myocardial apoptosis and infarction in rabbits with acute myocardial ischemia and reperfusion
Abstract.Effects of ischemic postconditioning on myocardial apoptosis and infarction in rabbits with acute myocardial ischemia and reperfusion[J].Journal of the Fourth Military Medical University,2002,23(18):1690-1693.
Authors:Abstract
Abstract:AIM To observe the effects of ischemic postconditioning (IPC) on cardiomyocyte apoptosis and myocardial infarct size in myocardial ischemia/reperfusion rabbits. METHODS Rabbit acute myocardial ischemia/reperfusion model was used and the heart was conditioned either pre-or post-myocardial ischemia followed by reperfusion. Cardiomyocyte apoptosis and myocardial infarct size were determined by in situ TDT-mediated dUTP nick end labeling (TUNEL) and Evan's blue-TTC dual staining, respectively. RESULTS Compared with those in the control group, both cardiomyocyte apoptotic index and myocardial infarct size were significantly reduced in IPC group apoptotic index: (15±7)% vs (30±12)% of control, P<0.05; infarction:(13±5)% vs (27±7)% of control, P<0.01, respectively]. No significant difference was found between ischemic preconditioning (IP) and IPC groups. CONCLUSION Ischemic postconditioning, like ischemic preconditioning, exerts an anti-apoptotic effect, which may contribute partly to the ultimate reduction of myocardial infarction in acute myocardial ischemia-reperfusion.
Keywords:myocardial ischemia-reperfusion  ischemic postconditioning  apoptosis  myocardial infarction  CLC number: R542  22      Document code: A
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