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柴胡及甘草酸对小鼠肝细胞凋亡的影响
引用本文:陈东鸿,贺平.柴胡及甘草酸对小鼠肝细胞凋亡的影响[J].第四军医大学学报,1998,19(3):279-280.
作者姓名:陈东鸿  贺平
作者单位:第四军医大学唐都医院药学部,第二军医大学东方肝胆外科研究所
摘    要:目的:研究柴胡(RB)及甘草酸(GL)对小鼠肝细胞凋亡的影响。方法:以肝/体重比,肝组织DNA含量,组织学改变及原位凋亡细胞TUNEL反应为指标,观察RB(12.0g/kg)及GL(50mg/kg)ip,1次/8h,对撇除苯巴比胺钠(PB)后引起的小鼠肝细胞凋亡的影响。结果:与对照组C相比,RB组小鼠肝/体重比及肝DNA含量无明显回落,组织学检查未见明显凋亡改变,TUNEL反应阴性,GL组小鼠肝/

关 键 词:柴胡  甘草酸  苯巴比妥钠  肝细胞凋亡

Effects of radix bupleuri and glycyrrhizin on the hepatocyte apoptosis in mice
CHEN Dong Hong ,LI Ping ,CHENG Jian Feng ,ZHONG Chong Hua ,HE Jian Rong ,TENG Shu Bao ,HE Ping Deparment of Pharmacy,Tangdu Hospital,Fourth Military Medical University,Xi'an ,Dong Fang Institute of Hepato biliary.Effects of radix bupleuri and glycyrrhizin on the hepatocyte apoptosis in mice[J].Journal of the Fourth Military Medical University,1998,19(3):279-280.
Authors:CHEN Dong Hong  LI Ping  CHENG Jian Feng  ZHONG Chong Hua  HE Jian Rong  TENG Shu Bao  HE Ping Deparment of Pharmacy  Tangdu Hospital  Fourth Military Medical University  Xi'an  Dong Fang Institute of Hepato biliary
Institution:CHEN Dong Hong 1,LI Ping 1,CHENG Jian Feng 1,ZHONG Chong Hua 1,HE Jian Rong 1,TENG Shu Bao 1,HE Ping 2 1Deparment of Pharmacy,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,2Dong Fang Institute of Hepato biliary
Abstract:Aim: The effects of radix bupleuri (RB) and glycyrrhizin(GL)on the hepatocyte apoptosis in mice were investigated. Methods: The ratio of liver/body weight, hepatic DNA content, morphological changes and TUNEL reaction (TdT mediated X dUTP nick labeling), as indices of evaluating hepatocyte apoptosis, were used to observe the effects of RB (12.0 g/kg,ip) and GL (50 mg/kg,ip) on the hepatocyte apoptosis caused by phenobarbital (PB) withdrawal. Results: The regression of hepatic DNA content and ratio of liver/body weight and typical hepatocyte apoptosis, which occurred in the mice as control after stopping PB, were not found in the mice treated with RB,and a negative result of TUNEL assay was presented. However, in the mice given GL the ratio of liver/body weight and hepatic DNA content regressed by 12.0% and 13.6%, respectively; with a positive reaction of TUNEL assay and the typical changes of hepatocyte apoptosis. Conclusion: The results showed that RB, but not GL, prevented hepatocyte apoptosis caused by PB withdrawal in mice.
Keywords:radix bupleuri  glycyrrhizin  phenobarbital  hepatocyte apoptosis  
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