视网膜母细胞瘤眼房水细胞荧光显微检查

Fluorescence microscopic examination of retinoblastoma cells in aqueous humor

The results of fluorescence microscopy on the cells of aqueous humor in 30 children with rctinoblastoma were presented . The aqueous aspirated through paracentesis of anterior chamber by using a small needle was put on a microslide and precipitated after centrifugation , stained with acridine orange and examined under fluorescence microscope . The tumor cells in the aqueous were easily identified by their orange - yellow color . Positive results were obtained in all patients (N=30). Among them the diagnosis of 15 patients was confirmed pathologically after surgery . In the other 15 patients , their eye balls were preserved and treated with HPD - PRT . No metastasis was found over the sites of paracentesis of the anterior chamber after 3 to 5 year's follow - up .The results indicate that fluorescence microscopic examination of the aqueous humor is a simple , safe and comparatively effective method with high positive rate for the diagnosis of retinoblastoma .