肝硬化患者肝组织中TIMP-1,TIMP-2的表达

目的 了解ＴＩＭＰ－１和ＴＩＭＰ－２在肝硬化患肝组织中的表达状态，探讨了ＴＩＭＰ－１和ＴＩＭＰ－２在肝硬化发病机制中的作用。方法 以抗ＴＩＭＰ－１和ＴＩＭＰ－２单克隆抗体（ＭｃＡｂ）为试剂，采用免疫组织化学法检测肝硬化患肝组织中ＴＩＭＰ－１，ＴＩＭＰ－２相关抗原的表达。结果 ３０例肝硬化患肝组织中ＴＩＭＰ－１和ＴＩＭＰ－２相关抗原表达的阳性率为１００％，ＴＩＭＰ－１阳性的肝组织中ＴＩＭＰ－２

Tissue inhibitors of metalloproteinase-1 and -2 (TIMP-1 and TIMP-2) related antigens expression in liver tissues of patients with liver cirrhosis

AIM To investigate the distribution of tissue inhibitors of metallproteinanses 1 and 2 (TIMP 1 and TIMP 2) liver cirrhosis tissue of patients and discuss the function of TIMP 1 and TIMP 2 in pathogenesis of liver cirrhosis. METHODS TIMP 1 or TIMP 2 related antigens were detected in paraffin embeded liver tissue of biopsy patients with liver cirrhosis by immunohistochemical method using specific monoclonal antibody (McAb). RESULTS Among 30 samples, 100% had been detected out TIMP 1 and TIMP 2 related antigen in the liver cirrhosis. Both expressions of TIMP 1 and TIMP 2 were positive, and expression of TIMP 1 was higher than TIMP 2. None of normal liver tissue was positive. The staining signal of TIMP 1 and TIMP 2 related antigen was mainly located in cytoplasm of liver cells. There was no signal in nuclear of liver cells. CONCLUSION These studies suggest that both TIMP 1 and TIMP 2 related antigens may exist in liver cirrhosis tissue of patients. They are correlated with development. They reduce excellular matrix (ECM) and lead to liver fibrosis and liver cirrhosis by restraining the activity of matrix metalloproteinase.