| SOCS3基因沉默对高脂餐大鼠脂质代谢紊乱的影响 |
| |
| 引用本文： | 刘红梅.SOCS3基因沉默对高脂餐大鼠脂质代谢紊乱的影响[J].第四军医大学学报,2009(24):2963-2965. |
| |
| 作者姓名： | 刘红梅 |
| |
| 作者单位： | 咸宁学院基础医学院生物化学教研室; |
| |
| 基金项目： | 湖北省自然科学基金(2006ABA322) |
| |
| 摘 要： | 目的：探讨通过RNA干扰技术下调SOCS3表达防治高脂餐大鼠代谢紊乱的作用.方法：取SD6wk龄大鼠45只,随机分为3组：普通饮食对照组（n=15）,下丘脑注射空白质粒,普通饲料喂养; 高脂饮食对照组（n=15）,下丘脑注射空白质粒,高脂饲料喂养; 实验组（n=15）,下丘脑注射shR-NA-SOCS3序列慢病毒重组体,高脂饲料喂养.每周测体质量1次.饲养8wk末,测空腹血糖和体质量,处死后立即取心脏血进行血清胰岛素、三酰甘油、总胆固醇、血清瘦素测定.结果：实验组体质量、三酰甘油、总胆固醇低于高脂饮食对照组（P〈0.01）,高于普通饮食对照组（P〈0.05）.实验组血清瘦素、血清胰岛素浓度低于高脂饮食对照组组（P〈0.01）,与普通饮食对照组比较无统计学差异.结论：下调SOCS3基因表达可以降低体质量、三酰甘油、血清胰岛素、总胆固醇及血清瘦素水平,对肥胖症、糖尿病及慢性并发症可能有预防和治疗作用.
|
| 关 键 词： | 肥胖症 瘦素抵抗 胰岛素抵抗 RNA干扰 SOCS3 |
Effects of NRAi-mediated gene silencing of SOCS3 on metabolic disturbance in high-fat diet rats |
| |
| LIU Hong-Mei.Effects of NRAi-mediated gene silencing of SOCS3 on metabolic disturbance in high-fat diet rats[J].Journal of the Fourth Military Medical University,2009(24):2963-2965. |
| |
| Authors: | LIU Hong-Mei |
| |
| Institution: | LIU Hong-Mei Department of Biochemistry,School of Basic Medical,Xianning University,Xianning 437100,China |
| |
| Abstract: | AIM:To study the effects of NRAi-mediated gene silencing of SOCS3 on metabolic disturbance in high-fat diet rats.METHODS:Forty five 6-week-old healthy Sprague-Dawley(SD) rats were adopted to our research and divided into 3 groups randomly.In the standard control group(n=15),the control lenti were stereotaxically injected into the hypothalamus of rats which then fed with standard chow;In the high-fat diet control group(n=15),the control lenti were stereotaxically injected into the hypothalamus of rats which ... |
| |
| Keywords: | obesity leptin resistance insulin resistance RNAi SOCS3 |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
