| 糖尿病大鼠海马胆碱乙酰转移酶表达与认知功能的相关性 |
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| 引用本文： | 张栋珉,肖谦.糖尿病大鼠海马胆碱乙酰转移酶表达与认知功能的相关性[J].第四军医大学学报,2009(22):2553-2556. |
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| 作者姓名： | 张栋珉 肖谦 |
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| 作者单位： | 重庆医科大学附属第一医院老年病科内分泌组; |
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| 基金项目： | 重庆市卫生局医学科研项目[渝卫科教2008(45)-2-79] |
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| 摘 要： | 目的：观察糖尿病大鼠海马胆碱乙酰转移酶（ChAT）mRNA和蛋白表达及其与认知功能的关系,探讨糖尿病脑病的发病机制.方法：38只SD雄性大鼠随机分为正常对照组（C组）和糖尿病组（D组）,腹腔注射链脲佐菌素建立糖尿病大鼠模型.建模成功后11wk用Morris水迷宫测试大鼠学习和记忆能力;RT-PCR,原位杂交法检测ChATmRNA表达;免疫组化,WesternBlot法检测ChAT蛋白表达.结果：D组大鼠学习和记忆能力明显减退,其逃避潜伏期时间延长;C组40.90±10.90与D组77.56±27.86相比较,差异具有统计学意义（P〈0.05）.穿越目标区域次数C组（3.93±0.44）次与D组（1.37±0.85）次相比较明显减少（P〈0.05）;中心区域停留时间百分率C组（5.41±0.97）%与D组（2.20±1.28）%相比较明显下降（P〈0.05）.海马ChATmRNA和ChAT蛋白表达C组0.48±0.03,0.55±0.02与D组0.37±0.01,0.33±0.01相比较均明显降低（P〈0.05）.结论：糖尿病大鼠海马ChATmRNA和蛋白低水平表达可能是糖尿病脑病的发病机制之一.
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| 关 键 词： | 糖尿病 胆碱乙酰转移酶 认知功能 |
Association between hippocampal Choline acetyltransferase expression and cognition in diabetes mellitus rats |
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| ZHANG Dong-Min,XIAO Qian Endocrinology Group.Association between hippocampal Choline acetyltransferase expression and cognition in diabetes mellitus rats[J].Journal of the Fourth Military Medical University,2009(22):2553-2556. |
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| Authors: | ZHANG Dong-Min XIAO Qian Endocrinology Group |
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| Institution: | ZHANG Dong-Min,XIAO Qian Endocrinology Group,Department of Geriatrics,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | AIM:To observe the relation between the expression of Choline acetyltransferase protein and mRNA of hippocampus and the cognitive function of diabetic rats and to explore the pathogenetic mechanism of diabetic encephalopathy.METHODS:Thirty-eight SD male rats were randomly divided into normal control group(group C)and diabetic model group(group D).Diabetes was inducedby a single peritoneal injection of streptozotocin.Eleven weeks later,learning and memory behaviors were investigated usinga spatial version of... |
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| Keywords: | diabetes mellitus choline acetyltransferase cognition |
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