| 人腹膜间皮细胞对卵巢癌SKOV3细胞黏附、迁移和侵袭力的影响 |
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| 引用本文： | 郭清,吴小华,范红伟,吉向丽.人腹膜间皮细胞对卵巢癌SKOV3细胞黏附、迁移和侵袭力的影响[J].第四军医大学学报,2009,30(8):726-729. |
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| 作者姓名： | 郭清 吴小华 范红伟 吉向丽 |
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| 作者单位： | 郭清,范红伟,吉向丽,GUO Qing,FAN Hong-Wei,JI Xiang-Li(河北医科大学第四医院妇产科,河北,石家庄,050011);吴小华,WU Xiao-Hua(河北医科大学第四医院妇产科,河北,石家庄,050011;白求恩国际和平医院妇产科,河北,石家庄,050051)
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| 摘 要： | 目的：探讨人腹膜间皮细胞（HPMC）对卵巢癌SK-OV3细胞黏附、迁移、侵袭力的影响及可能机制.方法：建立HPMC原代培养的方法;采用免疫细胞组织化学法鉴定原代HPMC;验证HPMC中部分细胞因子的表达;采用黏附、迁移及侵袭实验观察HPMC与卵巢癌SKOV3细胞相互作用时对SKOV3细胞转移力的影响,及其作用机制是否与CXCL12-CXCR4轴有关.结果：成功培养出了HPMC.鉴定分离出的细胞符合间皮细胞的特征.HPMC中表达CXCL12、间皮素,不表达CXCR4.SKOV3与HPMC共同培养组的吸光度、迁移和侵袭细胞数显著高于单独SKOV3培养组,差异有统计学意义（P〈0.05）.稳定高表达CXCR4的SKOV3细胞（SKOV3-CX-CR4-cDNA）与HPMC共同培养组的吸光度、迁移和侵袭的细胞数目显著高于其余3组,差异均有统计学意义（P〈0.05）.结论：HPMC与SKOV3细胞相互作用时,增强了SKOV3细胞黏附、迁移和侵袭力,其作用机制与CXCL12-CXCR4轴有关.
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| 关 键 词： | 卵巢肿瘤 SKOV3 原代培养 腹膜 间质细胞 黏附 迁移 侵袭 |
Effect of human peritoneal mesothelial cells on adhesion,migration and invasion of ovarian carcinoma SKOV3 cell line |
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| GUO Qing,WU Xiao-Hua,FAN Hong-Wei,JI Xiang-Li.Effect of human peritoneal mesothelial cells on adhesion,migration and invasion of ovarian carcinoma SKOV3 cell line[J].Journal of the Fourth Military Medical University,2009,30(8):726-729. |
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| Authors: | GUO Qing WU Xiao-Hua FAN Hong-Wei JI Xiang-Li |
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| Institution: | GUO Qing1,WU Xiao-Hua1,2,FAN Hong-Wei1,JI Xiang-Li11Department of Gynecology,Fourth Hospital,Hebei Medical University,Shijiazhuang 050011,China,2Department of Gynecology and Obstretics,Bethune International Peace Hospital,Shijiazhuang 050051 |
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| Abstract: | AIM:To explore the effect of human peritoneal mesothelial cells (HPMC) on the adhesion,migration and invasion of ovarian carcinoma SKOV3 cell line and the potential mechanism. METHODS:The primary culture method for HPMC was established. Isolated HPMC were identified and some cell factors expression were examined by immunohistochemical analysis. Adhesion assay,migration assay and invasion assay were used to evaluate the metastatic ability of SKOV3. The role of axis of CXCL12-CXCR4 in SKOV3 metastatic ability... |
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| Keywords: | SKOV3 |
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