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反义端粒酶RNA抑制肝癌细胞生长的机制
引用本文:张传山,王文亮,黄高晻,刘利,胡沛臻,马福成,朱晓慧.反义端粒酶RNA抑制肝癌细胞生长的机制[J].第四军医大学学报,2001,22(18):1637-1640.
作者姓名:张传山  王文亮  黄高晻  刘利  胡沛臻  马福成  朱晓慧
作者单位:1. 第四军医大学西京医院病理科
2. 第四军医大学西京医院血液内科
摘    要:目的:观察反义端粒酶RNA对SMMC-7721肝癌细胞系的作用,探讨抗端粒端治疗在原发性肝癌治疗中的意义。方法:采用脂质体介导的方式将反义端粒酶RNA真核表达载体pBBS212/hTP转入SMMC7721细胞中,经潮霉素筛选,克隆细胞株扩增鉴定后,采用TRAP-PCR-ELISA,FCM及电镜检测反义端粒酶RNA对转染细胞端粒酶活性和细胞凋亡的影响;同时进行裸鼠荷瘤生长实验观察。结果:TRAP-PCR-ELISA法检测细胞端粒酶活性的结果为:实验组吸光度值为0.980,对照组吸光度值为2.861;FCM检测细胞凋亡结果为:实验组13.8%,对照组未见有凋亡峰形成,电镜观察发现转染细胞表现出典型的凋亡细胞形态。裸鼠移植瘤生长实验发现,转染细胞生长明显受到抑制作用,说明反义端粒酶RNA显抑制SMMC7721细胞的端粒酶活性,并且对其有显的促凋亡作用。结论:反义端粒酶RNA能有效地抑制肝癌细胞端粒酶活性,同时显促进癌细胞凋亡。

关 键 词:肝肿瘤  端粒酶  脱噬作用  细胞周期
文章编号:1000-2790(2001)18-1637-04
修稿时间:2001年5月20日

Effect of antisense telomerase RNA on SMMC7721
ZHANG Chuan-Shan,WANG Wen-Liang,HUANG Gao-Sheng,LIU Li,HU Pei -Zhen,MA Fu-Cheng,ZHU Xiao-Hui.Effect of antisense telomerase RNA on SMMC7721[J].Journal of the Fourth Military Medical University,2001,22(18):1637-1640.
Authors:ZHANG Chuan-Shan  WANG Wen-Liang  HUANG Gao-Sheng  LIU Li  HU Pei -Zhen  MA Fu-Cheng  ZHU Xiao-Hui
Institution:ZHANG Chuan-Shan1,WANG Wen-Liang1,HUANG Gao-Sheng1,LIU Li2,HU Pei -Zhen1,MA Fu-Cheng1,ZHU Xiao-Hui1 1Department of Pathology,2Department of Hematology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China
Abstract:AIM To observe the effect of antisense telomerase R NA on SMMC7721 hepatocarcinoma cell line and to investigate the significance of anti-telomerase therapy for primary hepatoc arcinoma. METHODS pBBS212/hTR, antisense telomerase RNA gene ex pression vector, was intro-duced into SMMC7721 cells by using lipofectin mediation. After hygromycin screening and cloning, TRAP-PCR-ELISA a nd FCM were used to observe the effects of pBBS212/hTR on telomerase activity an d cell apoptosis. Then the animal model was established by transplanting cells (SMMC7721 cell strain transfected by pBBS212-hTR gene) into the abdomens of nude mice. RESULTS TRAP-PCR-ELISA showed tha t the experimental group registered 0.980 and the control group 2.861 in telomerase activity; FCM showed that the experimental group registered 13.8% in apoptosis, suggesting that antisense telomerase RNA had a significant inhibition of SMMC7721 telomerase activity and a marked promotion of cell apoptosis, and that in the meantime it could inhibit cell proliferation. CONCLUSION Inhibition of hepatocarcinoma telomerase activity and promotion of carcinoma cells' apoptosis through antisense telomerase RNA provide an approach to the treatment of hepatocarcinoma.
Keywords:liver neoplasms  telomerase  apoptosis  c ell cycle
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