壳聚糖和尿囊素预防外科术后腹腔粘连效果的观察

目的:对比观察壳聚糖及尿囊素对术后腹腔粘连的预防作用,并探讨两者合用的协同作用及机理. 方法:成年纯种家兔96只制成粘连动物模型,随机分为对照组(A)、壳聚糖组(B),尿囊素组(C)和壳聚糖 尿囊素联合用药组(D),每组24只. 观察家兔术后一般情况, 并于术后1 wk和2 wk剖腹观察其腹腔粘连情况,按程度分级,切取标本行HE染色,显微镜下观察. 结果:粘连动物模型建立切实可靠,B,C组和D组粘连等级与A组比较均有统计学意义(P<0.05),且D组效果明显优于B,C组(P<0.05). 病理观察联合用药组炎性反应及纤维化程度均轻于对照组. 结论:术中应用壳聚糖和尿囊素均有预防术后腹腔粘连的作用,两者合用可产生协同作用.

Preventive effect of chitosan and allantoin against post-surgical abdominal adhesions

AIM: To investigate the preventive effect of chitosan and allantoin against post-surgical abdominal adhesions and to study the synergetic effects of these two drugs and discuss the mechanism. METHODS: Ninety-six rabbits were chosen to establish intestinal adhesion models, which were randomly and evenly divided into control group (group A),chitosan treatment group (group B), allantoin treatment group (group C) and chitosan +allantoin treatment group (group D). Right after the operation, the situations of rabbits were observed. Then the rabbits were dissected to evaluate the degree of adhesions 1 and 2 weeks after the operation. Study the pathological changes under microscope. RESULTS: The models of adhesions were reliable. The adhesion degrees of group B, C and D were all significantly decreased compared with that of group A (P<0.05). Group D showed a better effect than group B and C (P<0.05). Group B, C and D could alleviate the inflammation reaction and the degree of fibrosis compared with group A. CONCLUSION: Treatment with chitosan and allantoin during operation could prevent post-surgical abdominal adhesions, and the two drugs had a synergetic effect.