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原癌基因表达在哮喘气道重塑中的作用
引用本文:刘颖格,戚好文,李焕章.原癌基因表达在哮喘气道重塑中的作用[J].第四军医大学学报,2001,22(5):418-421.
作者姓名:刘颖格  戚好文  李焕章
作者单位:第四军医大学西京医院呼吸内科,
摘    要:目的观察原癌基因表达在哮喘气道重逆中的作用。方法卵蛋白致敏豚鼠 建立哮喘模型。Dot-blot,North-ern-blot分子杂交及免疫组织化学技术观察豚鼠气道上皮,肺组织中原癌基因c-fos,c-myc,c-jun和c-sis的表达及其与气道重塑的关系。结果:正常豚鼠气道及肺组织中c-fos和c-myc mRNA无或很少表达,哮喘发作后,豚鼠气道上皮及肺组织c-fos和c-mycmRNA的表达明显增加;免疫组织化学染色显示Fos,Myc,Jun及Sis在政党豚鼠低水平表达,4种原癌基因产物表达增,国,阳性反应细胞主要分布于气道上皮细胞胞质、气道及细支气的上皮细胞胞核及浸润的炎症细胞中,病理结果显示气道粘膜及小鼠气管平滑肌周围淋巴细胞、嗜酸性粒细胞及中性粒细胞浸润,气道平滑肌显著增生,结论原部达在气道重逆过程中有重要作用。

关 键 词:原癌基因  哮喘  气道重塑  豚鼠  基因表达
文章编号:1000-2790(2001)05-0418-04
修稿时间:2000年1月14日

Proto-oncogenes expression in airway remodeling in asthma
LIU Ying-Ge,QI Hao-wen,LI Huan-zhang.Proto-oncogenes expression in airway remodeling in asthma[J].Journal of the Fourth Military Medical University,2001,22(5):418-421.
Authors:LIU Ying-Ge  QI Hao-wen  LI Huan-zhang
Abstract:AIM To explore the role of proto oncogenes in the process of airway remodeling in asthma. METHODS Guinea pigs were used as asthma models challenged by ovoglobulin. Dot blot, Northern blot molecular hybridization and immunohistochemistry techniques were used to detect the expression of c fos , c myc , c jun and c sis . RESULTS c fos and c myc mRNA could not be detected or expressed at very low level in the control group. They were greatly increased after the guinea pigs were challenged by ovoglobulin. 30 min after the challenge, the expressions of c fos and c myc mRNA reached the peak and returned to normal 4 h after the challenge. Immunohistochemistry study showed that Fos, Myc, Jun and Sis expressed at low level in control group and increased after ovoglobulin stimulation. Immunohistochemically positive cells lay in the plasma of airway epithelium, in cell nucleus of bronchial epithelium and in inflammatory cells. Pathologic studies showed smooth muscle thickened around bronchia and lymphocyte infiltration under mucosa or around bronchia smooth muscle. CONCLUSION Proto oncogenes expression plays an important role in the process of asthma airway remodeling.
Keywords:oncoges  asthma  airway remodeling  guinea pigs
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