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肝癌组织中TIMP-1和TIMP-2的表达意义
引用本文:谢玉梅,聂青和,周永兴,康文臻,朱晓慧,王映梅.肝癌组织中TIMP-1和TIMP-2的表达意义[J].第四军医大学学报,2001,22(19):1787-1790.
作者姓名:谢玉梅  聂青和  周永兴  康文臻  朱晓慧  王映梅
作者单位:1. 第四军医大学唐都医院传染科
2. 第四军医大学基础部病理学教研室
基金项目:中国博士后科学基金资助 [中博基 ( 1999) 10号 ]
摘    要:目的 了解TIMP-1和TIMP-2在肝癌组织中的表达状态,探讨TIMP-1和TIMP-2在肝癌组织生长、侵润及转移中所起的作用。方法 以抗TIMP-1和TIMP-2单克隆抗体(mAb)为试剂,采用免疫组织化学法检测原发性肝癌、肝高分化腺癌的肝组织中TIMP-1和TIMP-2的表达,并与10例正常肝组织做对照。结果 10例原发性肝癌患的肝组织中TIMP-1和TIMP-2蛋白表达的阳性率为100%,在癌组织及非癌组织中均有表达。癌组织中的TIMP-1和TIMP-2蛋白表达的强度比较为6例高于、4例低于周围的非癌组织(慢性肝炎及肝硬化组织),癌组织中TIMP-1和TIMP-2蛋白的表达呈散在性分布,但TIMP-1比TIMP-2表达强。9例肝高分化腺癌的腺癌组织中无TIMP-1和TIMP-2相关抗原的表达,但癌周组织的肝细胞有3例TIMP-1和TIMP-2蛋白表达为阳性。10例正常肝组织中TIMP-1和TIMP-2蛋白表达均为阴性。结论 TIMP-1和TIMP-2存在于原发性肝癌的癌组织中,其表达的部位与强度可能与癌的生长、浸润及转移有关。

关 键 词:肝细胞癌  金属蛋白酶组织抑制因子  细胞外基质  免疫组织化学  TIMP-1  TIMP-2
文章编号:1000-2790(2001)19-1787-04
修稿时间:2001年1月5日

TIMP-1 and -2 related antigens expression in liver carcinoma tissues
XIE Yu Mei ,NIE Qing He ,ZHOU Yong Xing ,KANG Wen Zhen ,ZHU Xiao Hui ,WANG Ying Mei.TIMP-1 and -2 related antigens expression in liver carcinoma tissues[J].Journal of the Fourth Military Medical University,2001,22(19):1787-1790.
Authors:XIE Yu Mei  NIE Qing He  ZHOU Yong Xing  KANG Wen Zhen  ZHU Xiao Hui  WANG Ying Mei
Institution:XIE Yu Mei 1,NIE Qing He 1,ZHOU Yong Xing 1,KANG Wen Zhen 1,ZHU Xiao Hui 2,WANG Ying Mei 2 1Department of Infectious Diseases,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China,2Department of Patholo
Abstract:AIM To investigate the distribution of tissue inhibitor of metalloproteinasas 1 (TIMP 1) and TIMP 2 in liver carcinoma tissues and discuss the function of TIMP 1 and TIMP 2 for growth, invasion, and metastasis of carcinomas of liver. METHODS The cellular distribution of TIMP 1 and TIMP 2 was studied in surgically removed human hepatocellular carcinomas and well differentiated adenocacinoma of liver samples by immunohistochemical method using monoclone antibody (mAb). RESULTS TIMP 1 and TIMP 2 protein were detected in all 10 samples of the hepatocellular carcinomas. Both TIMP 1 and TIMP 2 were positive in the cancer cells and noncarcerous cells, and the expression of TIMP 1 was stronger than that of TIMP 2. The expression of TIMP 1 and TIMP 2 in 6 samples was higher, and that in other 4 samples was lower than that in the surrounding noncancerous cells (chronic active hepatitis and liiver cirrhosis). The distribution of TIMP 1 and TIMP 2 in the cancer cells was dispersed. TIMP 1 and TIMP 2 protein were not detected in the well differentiated adenocarcinomal of liver. Among the 9 samples, TIMP 1 and TIMP 2 protein were positive in the surrounding noncancerous liver tissue of 3 samples (chronic active hepatitis). None of normal liver tissue is positive. CONCLUSION Both expression of TIMP 1 and TIMP 2 in primary carcinomas of liver may be related with the growth, invasion and metastasis of primary carcinomas of liver.
Keywords:hepatocellular carcinomas  well  differentiated adenocarcinoma of liver  tissue inhibitor of metalloproteinasas  extracellular matrix  matrix metalloproteinase  immunohistochemistry
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