脂质转染剂增强基因疫苗诱导的免疫应答效力

目的 研究脂质转染剂（ＬｉｐｏｆｅｃｔＡＭＩＮＥ）提高丙型肝炎病毒（ＨＣＶ）核心（Ｃ）基因疫苗的抗病毒免疫应答效力。方法 人工构建包含ＨＣＶ Ｃ基因片段的的真核表达载体ｐｃＤ－ＮＡＨＣＶ－Ｃ，在证实期可以在真核细胞中表达之后，将期用脂质转剂包裹，形成ＬｉｐｏｆｅｃｔＡＭＩＮＥ－ｐｃＤＮＡＨＣＶ－Ｃ脂质混合物，将该混合物或单纯ｐｃＤＮＡＨＣＶ－Ｃ直接注射ＢＡＬＢ／ｃ小鼠股四头肌，以空载体ｐｃＤＮＡ

LipofectAMINE coated hepatitis C virus core gene vaccine promotes the efficacy of immune responses

AIM To promote the efficacy of LipofectAMINE coated recombinant plasmid containing hepatitis C virus (HCV) core gene inductive immune responses. METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled with Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme-cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors. The expressed products could combine with patient serum of HCV C antibody positive, and HCV C antibody was time dependence, and HCV C antibody expressing value from encapsuled with LipofectAMINE HCV core gene vaccine-immunized mice were higher than from control mice ( P < 0.05 ). CONCLUSION Liposome technique in combination with gene vaccine can promote the efficacy of immune responses in BALB/c mice.