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单克隆抗体分析单纯疱疹病毒的抗原性
引用本文:高谦,汪美先,姜绍谆,马文煜,戴敦穗.单克隆抗体分析单纯疱疹病毒的抗原性[J].第四军医大学学报,1988(4).
作者姓名:高谦  汪美先  姜绍谆  马文煜  戴敦穗
作者单位:第四军医大学微生物学教研室 (高谦,汪美先,姜绍谆,马文煜),第一军医大学南方医院(戴敦穗)
摘    要:用15种抗HSV型共同性McAb相2种分别抗HSV-1和HSV-2的型特异性McAb,分析了43株HSV临床株的抗原性。间接免疫荧光试验和改良ELISA的分机结果表明,从宫颈炎患者分离出的15株HSV中,1株为HSV-1,14株为HSV-2;从口唇和眼部感染病变中分离出的28株HSV均为HSV-1.用15种型共同性McAb对HSV的抗原性分析表明43株HSV分离株中,22株(51.2%)可与所有型共同性McAb反应,21株(48.8%)仅与部分McAb反应。结果表明,HSV临床分离株间及不同的HSV参考株间在抗原性上有明显差异,其原因有待进一步研究。

关 键 词:单克隆抗体  单纯疱疹病毒  抗原性分析  荧光抗体技术  酶联免疫吸附试验

Antigenic Analysis of Herpes Simplex Virus with Monoclonal Antibodies
Gao Qian,et al..Antigenic Analysis of Herpes Simplex Virus with Monoclonal Antibodies[J].Journal of the Fourth Military Medical University,1988(4).
Authors:Gao Qian  
Institution:Gao Qian,et al. Department of Microbiology
Abstract:The antigenicity of 43 herpes simplex virus (HSV) isolates and 3 HSV reference strains was analysed by 15 anti-HSV type common McAbs and 2 type specific McAbs for HSV-1 or HSV-2 respectively. By an indirect immunoflucres cence test and a modified EL1SA, it was shown that 14 HSV strains isolated from cervicitis patients were typed as HSV-2 but 1 as HSV-1 and that 28 HSV isolates from head and face HSV infectious patients were typed as HSV-1. Among 43 HSV isolates, 22(51.2%) isolates reacted with all of 15 type common McAbs and 21(48.8%) isolates reacted only with a number of them. HSV-2 reference strain 333 reacted only with 7 of 15 type common McAbs and did not react with an anti-HSV-2 type specific McAb. These results suggest that there are obvious antigenic variances among HSV isolates and different HSV reference strains. The cause responsible for this antigenic variances needs to be investigated.
Keywords:monoclonal antibodies  herpes simplex virus  antigenic analysis  fluorescent antibody technic  enzyme-linked immunosorbent assay
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